Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract

Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chro...

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Main Authors:	Nurul ‘Izzati Shafie, Nurdiana Samsulrizal, Nurfarhana Ahmad Sopian, Mohamed Ali Rajion, Goh, Yong Meng, Mohd Mokrish Md. Ajat, Hafandi Ahmad
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/10350/ http://journalarticle.ukm.my/10350/ http://journalarticle.ukm.my/10350/1/44_1_16.pdf

id	ukm-10350
recordtype	eprints
spelling	ukm-103502017-04-21T09:47:11Z http://journalarticle.ukm.my/10350/ Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract Nurul ‘Izzati Shafie, Nurdiana Samsulrizal, Nurfarhana Ahmad Sopian, Mohamed Ali Rajion, Goh, Yong Meng Mohd Mokrish Md. Ajat, Hafandi Ahmad, Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chromatography-mass spectrometry (GC-MS). Five grams of leaf extract were extracted using various solvents for phytochemical screening, while another five grams of ethanolic leaf extract was subjected for GC-MS analysis where the mass spectra of the compounds detected were matched with the National Institute of Standards and Technology (NIST) library. The results showed the presence of flavonoids, condensed tannin, triterpenes and steroids in phytochemical screening tests, while GC-MS analysis revealed the presence of seven major compounds in the ethanolic leaf extract. The major component of A.excelsa ethanolic leaf extract was 9, 12, 15-octadecatrienoic acid (42.34%), followed by pentadecanoic acid, 14-methyl-, methyl ester (28.99%), phytol (10.63%), 9, 12, 15-octadecatrien-1-ol (5.37%), octadecanoic acid, methyl ester (4.36%), 9, 12-octadecadienoic acid, methyl ester (4.24%) and hexadecanoic acid, ethyl ester (4.06%). Therefore, the findings of this study form a basis for the characterization of the compounds and allow researchers to investigate the potential of this plant in treating diabetes mellitus. Penerbit Universiti Kebangsaan Malaysia 2015-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10350/1/44_1_16.pdf Nurul ‘Izzati Shafie, and Nurdiana Samsulrizal, and Nurfarhana Ahmad Sopian, and Mohamed Ali Rajion, and Goh, Yong Meng and Mohd Mokrish Md. Ajat, and Hafandi Ahmad, (2015) Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract. Malaysian Applied Biology, 44 (3). pp. 87-92. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=520&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chromatography-mass spectrometry (GC-MS). Five grams of leaf extract were extracted using various solvents for phytochemical screening, while another five grams of ethanolic leaf extract was subjected for GC-MS analysis where the mass spectra of the compounds detected were matched with the National Institute of Standards and Technology (NIST) library. The results showed the presence of flavonoids, condensed tannin, triterpenes and steroids in phytochemical screening tests, while GC-MS analysis revealed the presence of seven major compounds in the ethanolic leaf extract. The major component of A.excelsa ethanolic leaf extract was 9, 12, 15-octadecatrienoic acid (42.34%), followed by pentadecanoic acid, 14-methyl-, methyl ester (28.99%), phytol (10.63%), 9, 12, 15-octadecatrien-1-ol (5.37%), octadecanoic acid, methyl ester (4.36%), 9, 12-octadecadienoic acid, methyl ester (4.24%) and hexadecanoic acid, ethyl ester (4.06%). Therefore, the findings of this study form a basis for the characterization of the compounds and allow researchers to investigate the potential of this plant in treating diabetes mellitus.
format	Article
author	Nurul ‘Izzati Shafie, Nurdiana Samsulrizal, Nurfarhana Ahmad Sopian, Mohamed Ali Rajion, Goh, Yong Meng Mohd Mokrish Md. Ajat, Hafandi Ahmad,
spellingShingle	Nurul ‘Izzati Shafie, Nurdiana Samsulrizal, Nurfarhana Ahmad Sopian, Mohamed Ali Rajion, Goh, Yong Meng Mohd Mokrish Md. Ajat, Hafandi Ahmad, Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
author_facet	Nurul ‘Izzati Shafie, Nurdiana Samsulrizal, Nurfarhana Ahmad Sopian, Mohamed Ali Rajion, Goh, Yong Meng Mohd Mokrish Md. Ajat, Hafandi Ahmad,
author_sort	Nurul ‘Izzati Shafie,
title	Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
title_short	Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
title_full	Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
title_fullStr	Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
title_full_unstemmed	Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
title_sort	qualitative phytochemical screening and gc-ms profiling of azadirachta excelsa leaf extract
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/10350/ http://journalarticle.ukm.my/10350/ http://journalarticle.ukm.my/10350/1/44_1_16.pdf
first_indexed	2023-09-18T19:57:09Z
last_indexed	2023-09-18T19:57:09Z
_version_	1777406605732610048

Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract

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