Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women

Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model. However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the umbilical cord donors. Commercial HUVEC is commonly derived from Europe...

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Main Authors:	Raja Nor Suhaila, Sabreena Safuan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/10717/ http://journalarticle.ukm.my/10717/ http://journalarticle.ukm.my/10717/1/14%20Raja%20Nor%20Suhaila.pdf

id	ukm-10717
recordtype	eprints
spelling	ukm-107172017-10-07T15:23:42Z http://journalarticle.ukm.my/10717/ Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women Raja Nor Suhaila, Sabreena Safuan, Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model. However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the umbilical cord donors. Commercial HUVEC is commonly derived from European and Caucasian population which have different molecular characteristics from Asian women. This study aimed to optimize the isolation and culture condition of HUVEC using combinations of growth factors and extracellular matrix components so that the isolated HUVEC will purely represent the population under study. Umbilical cords were obtained from women post-labour. Different incubation times and digestive enzymes were used during endothelial cells isolation process. The culture conditions were optimized based on the coating materials and the media supplements. The results showed that 0.1% collagenase for 40 min incubation was the optimal isolation condition of HUVEC. HUVEC grown in 0.2% gelatin coated plate with 10% heat-inactivated fetal calf serum showed higher proliferative capacity and reduced cell death compared to other conditions (p<0.05). The results generated from this study provide a basic protocol of HUVEC isolation and culture conditions in order to generate working endothelial cell populations purely represent the Malaysian population. Penerbit Universiti Kebangsaan Malaysia 2017-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10717/1/14%20Raja%20Nor%20Suhaila.pdf Raja Nor Suhaila, and Sabreena Safuan, (2017) Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women. Sains Malaysiana, 46 (3). pp. 463-468. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num3_2017/contentsVol46num3_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model. However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the umbilical cord donors. Commercial HUVEC is commonly derived from European and Caucasian population which have different molecular characteristics from Asian women. This study aimed to optimize the isolation and culture condition of HUVEC using combinations of growth factors and extracellular matrix components so that the isolated HUVEC will purely represent the population under study. Umbilical cords were obtained from women post-labour. Different incubation times and digestive enzymes were used during endothelial cells isolation process. The culture conditions were optimized based on the coating materials and the media supplements. The results showed that 0.1% collagenase for 40 min incubation was the optimal isolation condition of HUVEC. HUVEC grown in 0.2% gelatin coated plate with 10% heat-inactivated fetal calf serum showed higher proliferative capacity and reduced cell death compared to other conditions (p<0.05). The results generated from this study provide a basic protocol of HUVEC isolation and culture conditions in order to generate working endothelial cell populations purely represent the Malaysian population.
format	Article
author	Raja Nor Suhaila, Sabreena Safuan,
spellingShingle	Raja Nor Suhaila, Sabreena Safuan, Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
author_facet	Raja Nor Suhaila, Sabreena Safuan,
author_sort	Raja Nor Suhaila,
title	Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
title_short	Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
title_full	Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
title_fullStr	Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
title_full_unstemmed	Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
title_sort	isolation methods and culture conditions of human umbilical vein endothelial cells from malaysian women
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/10717/ http://journalarticle.ukm.my/10717/ http://journalarticle.ukm.my/10717/1/14%20Raja%20Nor%20Suhaila.pdf
first_indexed	2023-09-18T19:58:16Z
last_indexed	2023-09-18T19:58:16Z
_version_	1777406676145537024

Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women

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