The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ
Firefly is famously known for the light emission from its abdominal light organ. Light produce by firefly is known as bioluminescence. Luciferase is the enzyme that catalyze the light emitting reaction that produce bioluminescence. Haematoxylin and Eosin staining were used to describe the histologic...
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Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia
2016
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| Online Access: | http://journalarticle.ukm.my/10972/ http://journalarticle.ukm.my/10972/ http://journalarticle.ukm.my/10972/1/16139-45753-1-PB.pdf |
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ukm-109722017-11-23T04:35:16Z http://journalarticle.ukm.my/10972/ The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ Nur Khairunnisa S., Nurul Wahida Othman, Norela Sulaiman, Firefly is famously known for the light emission from its abdominal light organ. Light produce by firefly is known as bioluminescence. Luciferase is the enzyme that catalyze the light emitting reaction that produce bioluminescence. Haematoxylin and Eosin staining were used to describe the histological structure of the light organ. Localization of luciferase was discovered by immunohistochemical staining with polyclonal anti-luciferase and DAB chromogen as secondary antibody. Microphotography of light organ tissues was taken by Carl Zeiss Axio Scope A1 photomicroscope. This study revealed that the luciferase enzyme located at the photocyte cytoplasm of photogenic layer. This finding is an initial step to fully understand the regulation of synchronous light production in P. tener. Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10972/1/16139-45753-1-PB.pdf Nur Khairunnisa S., and Nurul Wahida Othman, and Norela Sulaiman, (2016) The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ. Serangga, 21 (1). pp. 51-59. ISSN 1394-5130 http://ejournal.ukm.my/serangga/issue/view/881/showToc |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Kebangasaan Malaysia |
| building |
UKM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
Firefly is famously known for the light emission from its abdominal light organ. Light produce by firefly is known as bioluminescence. Luciferase is the enzyme that catalyze the light emitting reaction that produce bioluminescence. Haematoxylin and Eosin staining were used to describe the histological structure of the light organ. Localization of luciferase was discovered by immunohistochemical staining with polyclonal anti-luciferase and DAB chromogen as secondary antibody. Microphotography of light organ tissues was taken by Carl Zeiss Axio Scope A1 photomicroscope. This study revealed that the luciferase enzyme located at the photocyte cytoplasm of photogenic layer. This finding is an initial step to fully understand the regulation of synchronous light production in P. tener. |
| format |
Article |
| author |
Nur Khairunnisa S., Nurul Wahida Othman, Norela Sulaiman, |
| spellingShingle |
Nur Khairunnisa S., Nurul Wahida Othman, Norela Sulaiman, The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| author_facet |
Nur Khairunnisa S., Nurul Wahida Othman, Norela Sulaiman, |
| author_sort |
Nur Khairunnisa S., |
| title |
The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| title_short |
The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| title_full |
The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| title_fullStr |
The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| title_full_unstemmed |
The localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| title_sort |
localization of luciferase in pteroptyx tener (coleoptera: lampyridae) light organ |
| publisher |
Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia |
| publishDate |
2016 |
| url |
http://journalarticle.ukm.my/10972/ http://journalarticle.ukm.my/10972/ http://journalarticle.ukm.my/10972/1/16139-45753-1-PB.pdf |
| first_indexed |
2023-09-18T19:58:57Z |
| last_indexed |
2023-09-18T19:58:57Z |
| _version_ |
1777406719148687360 |