Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia

Chikungunya fever, an Aedes borne viral disease, is becoming a serious public health concern today since the first reported outbreak in Port Klang in 1998/99. Recently, more outbreaks were reported in Malaysia. Entomological investigations were conducted in Chikungunya virus cases localities in Peni...

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Main Authors:	Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L.
Format:	Article
Language:	English
Published:	Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/10992/ http://journalarticle.ukm.my/10992/ http://journalarticle.ukm.my/10992/1/16651-46813-1-SM.pdf

id	ukm-10992
recordtype	eprints
spelling	ukm-109922017-11-23T04:54:39Z http://journalarticle.ukm.my/10992/ Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L. Chikungunya fever, an Aedes borne viral disease, is becoming a serious public health concern today since the first reported outbreak in Port Klang in 1998/99. Recently, more outbreaks were reported in Malaysia. Entomological investigations were conducted in Chikungunya virus cases localities in Peninsular Malaysia which cover Johor, Negeri Sembilan, Melaka, Perak, Pahang and Selangor state in order to identify the vector responsible for transmitting the Chikungunya virus. The adult mosquitoes were collected using modified aspirator and sweep net methods, whereas water holding containers were inspected for larvae. Reverse transcriptase polymerase chain reaction (RT-PCR) were used as the detection of the virus. Positive samples were inoculated on the cell to isolate the virus. The most common species collected at the localities was Aedes albopictus followed by Culex quinquefasciatus, Aedes aegypti and Armigeres sp. Five pools (n=78) of female, adult Aedes albopictus collected from Tangkak, Johor were positive for the Chikungunya virus as detected by reverse transcription-polymerase chain reaction. Three isolates were obtained and grouped with Central/East African genotype. The presence of Chikungunya virus in wild Aedes albopictus indicated that this mosquito is the most likely vector responsible for the transmission of virus to humans in Johor during the outbreak. Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10992/1/16651-46813-1-SM.pdf Rozilawati H., and Mohd Masri S, and Zairi J., and Yahaya M.A., and Nazni W.A., and Lee, H.L. (2016) Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia. Serangga, 21 (2). pp. 171-181. ISSN 1394-5130 http://ejournal.ukm.my/serangga/issue/view/890
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Chikungunya fever, an Aedes borne viral disease, is becoming a serious public health concern today since the first reported outbreak in Port Klang in 1998/99. Recently, more outbreaks were reported in Malaysia. Entomological investigations were conducted in Chikungunya virus cases localities in Peninsular Malaysia which cover Johor, Negeri Sembilan, Melaka, Perak, Pahang and Selangor state in order to identify the vector responsible for transmitting the Chikungunya virus. The adult mosquitoes were collected using modified aspirator and sweep net methods, whereas water holding containers were inspected for larvae. Reverse transcriptase polymerase chain reaction (RT-PCR) were used as the detection of the virus. Positive samples were inoculated on the cell to isolate the virus. The most common species collected at the localities was Aedes albopictus followed by Culex quinquefasciatus, Aedes aegypti and Armigeres sp. Five pools (n=78) of female, adult Aedes albopictus collected from Tangkak, Johor were positive for the Chikungunya virus as detected by reverse transcription-polymerase chain reaction. Three isolates were obtained and grouped with Central/East African genotype. The presence of Chikungunya virus in wild Aedes albopictus indicated that this mosquito is the most likely vector responsible for the transmission of virus to humans in Johor during the outbreak.
format	Article
author	Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L.
spellingShingle	Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L. Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
author_facet	Rozilawati H., Mohd Masri S, Zairi J., Yahaya M.A., Nazni W.A., Lee, H.L.
author_sort	Rozilawati H.,
title	Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
title_short	Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
title_full	Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
title_fullStr	Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
title_full_unstemmed	Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia
title_sort	detection and isolation of chikungunya virus from field collected aedes albopictus skuse in selected sites, peninsular malaysia
publisher	Pusat Sistematik Serangga, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/10992/ http://journalarticle.ukm.my/10992/ http://journalarticle.ukm.my/10992/1/16651-46813-1-SM.pdf
first_indexed	2023-09-18T19:59:03Z
last_indexed	2023-09-18T19:59:03Z
_version_	1777406724926341120

Detection and isolation of chikungunya virus from field collected Aedes albopictus skuse in selected sites, Peninsular Malaysia

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