Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1

Nowadays, in extreme changing environments, development of submergence tolerance variety is necessary for ensuring crop production stability where, it is known that Malaysian commercial rice varieties such as MR219, MR220 and MR263 were severely susceptible to submergence. First step towards the dev...

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Main Authors:	Welland Cosmas Mojulat, Mohd Rafii Yusop, Mohd Razi Ismail, Abdul Shukor Juraimi, Abdul Rahim Harun, Fahim Ahmed, Tanweer, Fatah Abro, Latif, Md Abdul
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11027/ http://journalarticle.ukm.my/11027/ http://journalarticle.ukm.my/11027/1/02%20Welland%20Cosmas%20Mojulat.pdf

id	ukm-11027
recordtype	eprints
spelling	ukm-110272017-11-27T09:19:52Z http://journalarticle.ukm.my/11027/ Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1 Welland Cosmas Mojulat, Mohd Rafii Yusop, Mohd Razi Ismail, Abdul Shukor Juraimi, Abdul Rahim Harun, Fahim Ahmed, Tanweer, Fatah Abro Latif, Md Abdul Nowadays, in extreme changing environments, development of submergence tolerance variety is necessary for ensuring crop production stability where, it is known that Malaysian commercial rice varieties such as MR219, MR220 and MR263 were severely susceptible to submergence. First step towards the development of submergence tolerance variety starts with the breeding program by crossing MR263 and Swarna-Sub1. Marker-assisted selection (MAS) was carried out through the utilization of simple sequence repeats (SSR) markers, considering its reliability as pre-selection tools to conduct this research. F1 generations plants were confirmed by tightly linked markers. In case of background study, out of 180 SSR markers, 38 were found polymorphic between two parents. Association of molecular markers and submergence tolerance were determined using Chi-square test. MR263 × Swarna-Sub1 F2 lines were tested for Sub1 gene conformation using the markers RM8300 and RM219. These markers showed a good fit to the expected marker segregation ratio (1:2:1) in a Mendelian single gene model (DF=1.0, p≤0.05). Eleven homozygous lines with Sub1 gene out of 256 were selected for future development of submergence tolerant varieties. Eleven lines were selected based on phenotypic study and agronomic performance. Penerbit Universiti Kebangsaan Malaysia 2017-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11027/1/02%20Welland%20Cosmas%20Mojulat.pdf Welland Cosmas Mojulat, and Mohd Rafii Yusop, and Mohd Razi Ismail, and Abdul Shukor Juraimi, and Abdul Rahim Harun, and Fahim Ahmed, and Tanweer, Fatah Abro and Latif, Md Abdul (2017) Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1. Sains Malaysiana, 46 (4). pp. 521-528. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num4_2017/contentsVol46num4_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Nowadays, in extreme changing environments, development of submergence tolerance variety is necessary for ensuring crop production stability where, it is known that Malaysian commercial rice varieties such as MR219, MR220 and MR263 were severely susceptible to submergence. First step towards the development of submergence tolerance variety starts with the breeding program by crossing MR263 and Swarna-Sub1. Marker-assisted selection (MAS) was carried out through the utilization of simple sequence repeats (SSR) markers, considering its reliability as pre-selection tools to conduct this research. F1 generations plants were confirmed by tightly linked markers. In case of background study, out of 180 SSR markers, 38 were found polymorphic between two parents. Association of molecular markers and submergence tolerance were determined using Chi-square test. MR263 × Swarna-Sub1 F2 lines were tested for Sub1 gene conformation using the markers RM8300 and RM219. These markers showed a good fit to the expected marker segregation ratio (1:2:1) in a Mendelian single gene model (DF=1.0, p≤0.05). Eleven homozygous lines with Sub1 gene out of 256 were selected for future development of submergence tolerant varieties. Eleven lines were selected based on phenotypic study and agronomic performance.
format	Article
author	Welland Cosmas Mojulat, Mohd Rafii Yusop, Mohd Razi Ismail, Abdul Shukor Juraimi, Abdul Rahim Harun, Fahim Ahmed, Tanweer, Fatah Abro Latif, Md Abdul
spellingShingle	Welland Cosmas Mojulat, Mohd Rafii Yusop, Mohd Razi Ismail, Abdul Shukor Juraimi, Abdul Rahim Harun, Fahim Ahmed, Tanweer, Fatah Abro Latif, Md Abdul Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
author_facet	Welland Cosmas Mojulat, Mohd Rafii Yusop, Mohd Razi Ismail, Abdul Shukor Juraimi, Abdul Rahim Harun, Fahim Ahmed, Tanweer, Fatah Abro Latif, Md Abdul
author_sort	Welland Cosmas Mojulat,
title	Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
title_short	Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
title_full	Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
title_fullStr	Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
title_full_unstemmed	Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1
title_sort	analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from mr263 × swarna-sub1
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11027/ http://journalarticle.ukm.my/11027/ http://journalarticle.ukm.my/11027/1/02%20Welland%20Cosmas%20Mojulat.pdf
first_indexed	2023-09-18T19:59:08Z
last_indexed	2023-09-18T19:59:08Z
_version_	1777406730046537728

Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × Swarna-Sub1

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