In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

Temporary immersion system, Recipient for Automated Temporary Immersion® (RITA®), is one of the innovative systems that allow the production of large number of somatic embryos or plantlets via in vitro plant propagation. It has been widely used to avoid associated problems such as low multiplication...

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Main Authors: Noor Madihah Mohd, Hafsah Ja'afar, Dhiya Dalila Zawawi, Nadiawati Alias
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/11103/
http://journalarticle.ukm.my/11103/
http://journalarticle.ukm.my/11103/1/08%20Noor%20Madihah.pdf
id ukm-11103
recordtype eprints
spelling ukm-111032017-12-18T08:45:04Z http://journalarticle.ukm.my/11103/ In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA® Noor Madihah Mohd, Hafsah Ja'afar, Dhiya Dalila Zawawi, Nadiawati Alias, Temporary immersion system, Recipient for Automated Temporary Immersion® (RITA®), is one of the innovative systems that allow the production of large number of somatic embryos or plantlets via in vitro plant propagation. It has been widely used to avoid associated problems such as low multiplication rate of somatic embryos and hyperhydricity during plant propagation. Thus, an attempt was made to investigate optimal parameters such as immersion time and immersion frequency, for the multiplication of direct somatic embryogenesis from cotyledon culture of Eurycoma longifolia Jack using RITA®. Four periods of immersion time (1, 5, 10 and 15 min every 4 h) were evaluated for the efficiency in somatic embryos multiplication. In order to optimize repetitive somatic embryogenesis, three different immersion frequencies (5 min immersion every 2, 4 and 8 h) were applied. The highest number of secondary embryos (69.67 ± 9.73) was found significant when immersing the globular, primary embryos for 5 min every 4 h as compared to other immersion time tested. The secondary somatic embryos obtained in this study could be further used for the development of plantlet regeneration of E. longifolia. Penerbit Universiti Kebangsaan Malaysia 2017-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11103/1/08%20Noor%20Madihah.pdf Noor Madihah Mohd, and Hafsah Ja'afar, and Dhiya Dalila Zawawi, and Nadiawati Alias, (2017) In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®. Sains Malaysiana, 46 (6). pp. 897-902. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid46bil6_2017/KandunganJilid46Bil6_2017.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Temporary immersion system, Recipient for Automated Temporary Immersion® (RITA®), is one of the innovative systems that allow the production of large number of somatic embryos or plantlets via in vitro plant propagation. It has been widely used to avoid associated problems such as low multiplication rate of somatic embryos and hyperhydricity during plant propagation. Thus, an attempt was made to investigate optimal parameters such as immersion time and immersion frequency, for the multiplication of direct somatic embryogenesis from cotyledon culture of Eurycoma longifolia Jack using RITA®. Four periods of immersion time (1, 5, 10 and 15 min every 4 h) were evaluated for the efficiency in somatic embryos multiplication. In order to optimize repetitive somatic embryogenesis, three different immersion frequencies (5 min immersion every 2, 4 and 8 h) were applied. The highest number of secondary embryos (69.67 ± 9.73) was found significant when immersing the globular, primary embryos for 5 min every 4 h as compared to other immersion time tested. The secondary somatic embryos obtained in this study could be further used for the development of plantlet regeneration of E. longifolia.
format Article
author Noor Madihah Mohd,
Hafsah Ja'afar,
Dhiya Dalila Zawawi,
Nadiawati Alias,
spellingShingle Noor Madihah Mohd,
Hafsah Ja'afar,
Dhiya Dalila Zawawi,
Nadiawati Alias,
In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
author_facet Noor Madihah Mohd,
Hafsah Ja'afar,
Dhiya Dalila Zawawi,
Nadiawati Alias,
author_sort Noor Madihah Mohd,
title In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
title_short In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
title_full In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
title_fullStr In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
title_full_unstemmed In vitro somatic embryos multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®
title_sort in vitro somatic embryos multiplication of eurycoma longifolia jack using temporary immersion system rita®
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/11103/
http://journalarticle.ukm.my/11103/
http://journalarticle.ukm.my/11103/1/08%20Noor%20Madihah.pdf
first_indexed 2023-09-18T19:59:21Z
last_indexed 2023-09-18T19:59:21Z
_version_ 1777406743797563392

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