A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.

Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs)...

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Main Authors:	Zou, Li-Juan, Yang, Jing-Tian, Wu, Qing-Gui
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11137/ http://journalarticle.ukm.my/11137/ http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf

id	ukm-11137
recordtype	eprints
spelling	ukm-111372017-12-21T05:18:11Z http://journalarticle.ukm.my/11137/ A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L. Zou, Li-Juan Yang, Jing-Tian Wu, Qing-Gui Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for callus induction and organogenesis. Zeatin (ZT), thidiazuron (TDZ), kinetin (Kin) and indole-3-acetic acid (IAA) were found to induce multiple shoots. Shoot organogenesis was induced in the five explants. The highest mean for number of shoots per petioles (31.54±5.76) and rooted hypocotyls (44.00±1.51) with a 100% induction rate was obtained on Murashige and Skoog (MS) medium fortified with 0.4 mg/L IAA and 3.0 mg/L ZT. MS medium containing 0.4 mg/L IAA and 3.0 mg/L TDZ was found to be optimal for shoot regeneration of roots, leaves and nodal segments. The highest regeneration frequency (100%) with mean numbers of shoots equal to 38.77±6.87 for roots, 42.73±7.75 for leaves and 56.73±7.98 for nodal segments was produced. Regenerated shoots rooted effectively on half-strength MS medium and acclimatized successfully in soil with a 100% survival rate and normal growth. The protocol can be used for the large-scale propagation of S. nigrum to meet the increasing demand of commercial cultivation. Penerbit Universiti Kebangsaan Malaysia 2017-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf Zou, Li-Juan and Yang, Jing-Tian and Wu, Qing-Gui (2017) A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L. Sains Malaysiana, 46 (8). pp. 1183-1189. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid46bil8_2017/KandunganJilid46Bil8_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for callus induction and organogenesis. Zeatin (ZT), thidiazuron (TDZ), kinetin (Kin) and indole-3-acetic acid (IAA) were found to induce multiple shoots. Shoot organogenesis was induced in the five explants. The highest mean for number of shoots per petioles (31.54±5.76) and rooted hypocotyls (44.00±1.51) with a 100% induction rate was obtained on Murashige and Skoog (MS) medium fortified with 0.4 mg/L IAA and 3.0 mg/L ZT. MS medium containing 0.4 mg/L IAA and 3.0 mg/L TDZ was found to be optimal for shoot regeneration of roots, leaves and nodal segments. The highest regeneration frequency (100%) with mean numbers of shoots equal to 38.77±6.87 for roots, 42.73±7.75 for leaves and 56.73±7.98 for nodal segments was produced. Regenerated shoots rooted effectively on half-strength MS medium and acclimatized successfully in soil with a 100% survival rate and normal growth. The protocol can be used for the large-scale propagation of S. nigrum to meet the increasing demand of commercial cultivation.
format	Article
author	Zou, Li-Juan Yang, Jing-Tian Wu, Qing-Gui
spellingShingle	Zou, Li-Juan Yang, Jing-Tian Wu, Qing-Gui A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
author_facet	Zou, Li-Juan Yang, Jing-Tian Wu, Qing-Gui
author_sort	Zou, Li-Juan
title	A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_short	A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_full	A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_fullStr	A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_full_unstemmed	A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_sort	protocol for rapid and high-frequency in vitro propagation of solanum nigrum l.
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11137/ http://journalarticle.ukm.my/11137/ http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf
first_indexed	2023-09-18T19:59:28Z
last_indexed	2023-09-18T19:59:28Z
_version_	1777406751608406016

A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.

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