Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica

Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica

Isoflavonoids are the main compound in White Kwao Krua (Pueraria mirifica), which is an effective folk medicinal plant endemic to Thailand. It has been widely used for improving human physical and treating diseases. There are substances with estrogenic activities have been isolated from P. mirifica,...

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Main Authors: Li, Xu, Ruethaithip Kaopong, Sureeporn Nualkaew, Arthiya Chullasara, Amornrat Phongdara
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/11375/
http://journalarticle.ukm.my/11375/
http://journalarticle.ukm.my/11375/1/18%20Li%20Xu%20.pdf
id ukm-11375
recordtype eprints
spelling ukm-113752018-02-15T05:17:11Z http://journalarticle.ukm.my/11375/ Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica Li, Xu Ruethaithip Kaopong, Sureeporn Nualkaew, Arthiya Chullasara, Amornrat Phongdara, Isoflavonoids are the main compound in White Kwao Krua (Pueraria mirifica), which is an effective folk medicinal plant endemic to Thailand. It has been widely used for improving human physical and treating diseases. There are substances with estrogenic activities have been isolated from P. mirifica, such as puerarin, daidzein and genistein. Isoflavone synthase (IFS) is one of the key enzymes in Leguminous plants to convert liquiritigenin, liquiritigenin C-glucoside and naringenin chalcone to isoflavonoids. The aim of this research was to enhance the production of isoflavonoids by metabolic engineering. Transgenic plants were constructed by introducing P450 gene (EgP450) which is similar to IFS from oil palm (Elaeis guineensis), into P. mirifica by a biolistic method. After the transgenic plants had proved successfully, isoflavonoids of each group plants were determined by HPLC. The contents of daidzein and genistein in transgenic plants were higher than the control plants. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11375/1/18%20Li%20Xu%20.pdf Li, Xu and Ruethaithip Kaopong, and Sureeporn Nualkaew, and Arthiya Chullasara, and Amornrat Phongdara, (2017) Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica. Sains Malaysiana, 46 (9). pp. 1491-1498. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Isoflavonoids are the main compound in White Kwao Krua (Pueraria mirifica), which is an effective folk medicinal plant endemic to Thailand. It has been widely used for improving human physical and treating diseases. There are substances with estrogenic activities have been isolated from P. mirifica, such as puerarin, daidzein and genistein. Isoflavone synthase (IFS) is one of the key enzymes in Leguminous plants to convert liquiritigenin, liquiritigenin C-glucoside and naringenin chalcone to isoflavonoids. The aim of this research was to enhance the production of isoflavonoids by metabolic engineering. Transgenic plants were constructed by introducing P450 gene (EgP450) which is similar to IFS from oil palm (Elaeis guineensis), into P. mirifica by a biolistic method. After the transgenic plants had proved successfully, isoflavonoids of each group plants were determined by HPLC. The contents of daidzein and genistein in transgenic plants were higher than the control plants.
format Article
author Li, Xu
Ruethaithip Kaopong,
Sureeporn Nualkaew,
Arthiya Chullasara,
Amornrat Phongdara,
spellingShingle Li, Xu
Ruethaithip Kaopong,
Sureeporn Nualkaew,
Arthiya Chullasara,
Amornrat Phongdara,
Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
author_facet Li, Xu
Ruethaithip Kaopong,
Sureeporn Nualkaew,
Arthiya Chullasara,
Amornrat Phongdara,
author_sort Li, Xu
title Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
title_short Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
title_full Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
title_fullStr Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
title_full_unstemmed Expression and functional analysis of a transgenic cytochrome P450 monooxygenase in Pueraria mirifica
title_sort expression and functional analysis of a transgenic cytochrome p450 monooxygenase in pueraria mirifica
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/11375/
http://journalarticle.ukm.my/11375/
http://journalarticle.ukm.my/11375/1/18%20Li%20Xu%20.pdf
first_indexed 2023-09-18T20:00:07Z
last_indexed 2023-09-18T20:00:07Z
_version_ 1777406792142159872

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