Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1

In a previous study, the gene EgP450 that encodes the proteins of 505 amino acids was isolated from oil palm. The recombinant protein EgP450 is bound to phenylurea-like herbicides which detoxify the substance. Aflatoxin B1 (AFB1), a mycotoxin produced by Aspergillus sp., is another toxic compound th...

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Main Authors:	Sureeporn Nualkaew, Hirun Saelim, Danai Tiwawech, Tanate Panrat Imran Parvez, Amornrat Phongdara
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11376/ http://journalarticle.ukm.my/11376/ http://journalarticle.ukm.my/11376/1/19%20Sureeporn.pdf

id	ukm-11376
recordtype	eprints
spelling	ukm-113762018-02-15T05:18:07Z http://journalarticle.ukm.my/11376/ Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1 Sureeporn Nualkaew, Hirun Saelim, Danai Tiwawech, Tanate Panrat Imran Parvez, Amornrat Phongdara, In a previous study, the gene EgP450 that encodes the proteins of 505 amino acids was isolated from oil palm. The recombinant protein EgP450 is bound to phenylurea-like herbicides which detoxify the substance. Aflatoxin B1 (AFB1), a mycotoxin produced by Aspergillus sp., is another toxic compound that is known to cause acute toxic effects and act as a hepatocarcinogenic agent. This study aimed to examine the role of EgP450 enzyme in mycotoxin bioactivation in human mesenchymal stem cells (hMSCs). Docking analysis showed that EgP450 is bound to the group of carcinogens, which includes AFB1, n-(2-fluorenyl) acetamide, n-n-butyl-n-butan-4-ol-nitrosamine, n-nitrosodiethylamine, n-nitrosodiethylamine and n-nitrosodimethylamine. An in vivo aflatoxin toxicity test on hMSCs and AFB1 induces the expression of Bmi-1 which is one of the markers for the development of cancer. The presence of EgP450 at 0.15 μg/mL could reduced the Bmi-1 expression in AFB1 induced cells. Moreover, this protein also showed some antioxidant activity. These results exhibited the enormous potential of EgP450 in the detoxification processes. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11376/1/19%20Sureeporn.pdf Sureeporn Nualkaew, and Hirun Saelim, and Danai Tiwawech, and Tanate Panrat Imran Parvez, and Amornrat Phongdara, (2017) Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1. Sains Malaysiana, 46 (9). pp. 1499-1503. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In a previous study, the gene EgP450 that encodes the proteins of 505 amino acids was isolated from oil palm. The recombinant protein EgP450 is bound to phenylurea-like herbicides which detoxify the substance. Aflatoxin B1 (AFB1), a mycotoxin produced by Aspergillus sp., is another toxic compound that is known to cause acute toxic effects and act as a hepatocarcinogenic agent. This study aimed to examine the role of EgP450 enzyme in mycotoxin bioactivation in human mesenchymal stem cells (hMSCs). Docking analysis showed that EgP450 is bound to the group of carcinogens, which includes AFB1, n-(2-fluorenyl) acetamide, n-n-butyl-n-butan-4-ol-nitrosamine, n-nitrosodiethylamine, n-nitrosodiethylamine and n-nitrosodimethylamine. An in vivo aflatoxin toxicity test on hMSCs and AFB1 induces the expression of Bmi-1 which is one of the markers for the development of cancer. The presence of EgP450 at 0.15 μg/mL could reduced the Bmi-1 expression in AFB1 induced cells. Moreover, this protein also showed some antioxidant activity. These results exhibited the enormous potential of EgP450 in the detoxification processes.
format	Article
author	Sureeporn Nualkaew, Hirun Saelim, Danai Tiwawech, Tanate Panrat Imran Parvez, Amornrat Phongdara,
spellingShingle	Sureeporn Nualkaew, Hirun Saelim, Danai Tiwawech, Tanate Panrat Imran Parvez, Amornrat Phongdara, Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
author_facet	Sureeporn Nualkaew, Hirun Saelim, Danai Tiwawech, Tanate Panrat Imran Parvez, Amornrat Phongdara,
author_sort	Sureeporn Nualkaew,
title	Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
title_short	Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
title_full	Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
title_fullStr	Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
title_full_unstemmed	Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1
title_sort	role of cytochrome p450 monooxygenase in the bioactivation of aflatoxin b1
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11376/ http://journalarticle.ukm.my/11376/ http://journalarticle.ukm.my/11376/1/19%20Sureeporn.pdf
first_indexed	2023-09-18T20:00:07Z
last_indexed	2023-09-18T20:00:07Z
_version_	1777406792296300544

Role of cytochrome P450 monooxygenase in the bioactivation of aflatoxin B1

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