Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor

A kinetic model of bacterial growth and metabolite production can adequately explain the trends and interaction of important parameters in a fermentation process. Production of surfactin by two bacterial strains, namely, Bacillus subtilis MSH1 and Bacillus subtilis ATCC 21322, in a 5 L bioreactor wa...

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Main Authors:	Muhammad Qadri Effendy Mubarak, Siti Hajar Mohamad Jufri, Shikh Mohd Shahrul Nizan Shikh Zahar, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Mohd Hafez Mohd Isa
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11381/ http://journalarticle.ukm.my/11381/ http://journalarticle.ukm.my/11381/1/24%20Muhammad%20Qadri.pdf

id	ukm-11381
recordtype	eprints
spelling	ukm-113812018-02-15T05:25:35Z http://journalarticle.ukm.my/11381/ Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor Muhammad Qadri Effendy Mubarak, Siti Hajar Mohamad Jufri, Shikh Mohd Shahrul Nizan Shikh Zahar, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Mohd Hafez Mohd Isa, A kinetic model of bacterial growth and metabolite production can adequately explain the trends and interaction of important parameters in a fermentation process. Production of surfactin by two bacterial strains, namely, Bacillus subtilis MSH1 and Bacillus subtilis ATCC 21322, in a 5 L bioreactor was investigated using Cooper’s media with 4% (v/v) glucose. The present kinetic study was carried out in order to determine the correlation between microbial cell growth, surfactin production and glucose consumption. Batch fermentation was performed by cultivation of each selected strain in a bioreactor at 30°C for 55 h. The experimental results showed production of surfactin in the culture medium after 5 and 10 h of incubation for B. subtilis ATCC 21332 and B. subtilis MSH1, respectively, at which the bacterial cells were at an early stage of the log phase. The maximum concentration of surfactin (Pmax) achieved by B. subtilis MSH1 and B. subtilis ATCC 21332 was 226.17 and 447.26 mg/L, respectively. The kinetic study of bacterial cell growth of both strains indicated that B. subtilis MSH1 had a specific growth rate (μmax) of 0.224 h-1 and attained a maximum biomass concentration (Xmax) as high as 2.90 g/L after 28 h of fermentation, while B. subtilis ATCC 21332, with μmax of 0.087 h-1, attained an Xmax of 2.62 g/L after 45 h of incubation. B. subtilis MSH1 showed higher growth kinetics, thus exhibited higher values of μmax and Xmax compared with B. subtilis ATCC 21332 under identical fermentation conditions. The Pmax achieved by B. subtilis ATCC 21332 was 447.26 mg/L, two times higher than that achieved by B. subtilis MSH1 (226.17 mg/L). The results obtained provide kinetics information including values of Pmax, μmax and Xmax for better understanding of interactions of bacterial cell growth and glucose consumption towards surfactin production by a commercial strain of B. subtilis ATCC 21332 and a local isolate of B. subtilis MSH1. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11381/1/24%20Muhammad%20Qadri.pdf Muhammad Qadri Effendy Mubarak, and Siti Hajar Mohamad Jufri, and Shikh Mohd Shahrul Nizan Shikh Zahar, and Mohd. Sahaid Kalil, and Aidil Abdul Hamid, and Mohd Hafez Mohd Isa, (2017) Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor. Sains Malaysiana, 46 (9). pp. 1541-1548. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A kinetic model of bacterial growth and metabolite production can adequately explain the trends and interaction of important parameters in a fermentation process. Production of surfactin by two bacterial strains, namely, Bacillus subtilis MSH1 and Bacillus subtilis ATCC 21322, in a 5 L bioreactor was investigated using Cooper’s media with 4% (v/v) glucose. The present kinetic study was carried out in order to determine the correlation between microbial cell growth, surfactin production and glucose consumption. Batch fermentation was performed by cultivation of each selected strain in a bioreactor at 30°C for 55 h. The experimental results showed production of surfactin in the culture medium after 5 and 10 h of incubation for B. subtilis ATCC 21332 and B. subtilis MSH1, respectively, at which the bacterial cells were at an early stage of the log phase. The maximum concentration of surfactin (Pmax) achieved by B. subtilis MSH1 and B. subtilis ATCC 21332 was 226.17 and 447.26 mg/L, respectively. The kinetic study of bacterial cell growth of both strains indicated that B. subtilis MSH1 had a specific growth rate (μmax) of 0.224 h-1 and attained a maximum biomass concentration (Xmax) as high as 2.90 g/L after 28 h of fermentation, while B. subtilis ATCC 21332, with μmax of 0.087 h-1, attained an Xmax of 2.62 g/L after 45 h of incubation. B. subtilis MSH1 showed higher growth kinetics, thus exhibited higher values of μmax and Xmax compared with B. subtilis ATCC 21332 under identical fermentation conditions. The Pmax achieved by B. subtilis ATCC 21332 was 447.26 mg/L, two times higher than that achieved by B. subtilis MSH1 (226.17 mg/L). The results obtained provide kinetics information including values of Pmax, μmax and Xmax for better understanding of interactions of bacterial cell growth and glucose consumption towards surfactin production by a commercial strain of B. subtilis ATCC 21332 and a local isolate of B. subtilis MSH1.
format	Article
author	Muhammad Qadri Effendy Mubarak, Siti Hajar Mohamad Jufri, Shikh Mohd Shahrul Nizan Shikh Zahar, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Mohd Hafez Mohd Isa,
spellingShingle	Muhammad Qadri Effendy Mubarak, Siti Hajar Mohamad Jufri, Shikh Mohd Shahrul Nizan Shikh Zahar, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Mohd Hafez Mohd Isa, Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
author_facet	Muhammad Qadri Effendy Mubarak, Siti Hajar Mohamad Jufri, Shikh Mohd Shahrul Nizan Shikh Zahar, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Mohd Hafez Mohd Isa,
author_sort	Muhammad Qadri Effendy Mubarak,
title	Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
title_short	Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
title_full	Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
title_fullStr	Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
title_full_unstemmed	Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor
title_sort	kinetics of surfactin production by bacillus subtilis in a 5 l stirred-tank bioreactor
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11381/ http://journalarticle.ukm.my/11381/ http://journalarticle.ukm.my/11381/1/24%20Muhammad%20Qadri.pdf
first_indexed	2023-09-18T20:00:11Z
last_indexed	2023-09-18T20:00:11Z
_version_	1777406795925422080

Kinetics of surfactin production by Bacillus subtilis in a 5 L stirred-tank bioreactor

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