Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus

The viability of fermentation process is very much depends on the use of a cheap carbon source from lignocellulosic materials. It needs to hydrolyse into simple and readily metabolize carbohydrate using cellulase enzyme. Pycnoporus sanguineus has been able to produce cellulase enzyme with high enzym...

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Main Authors:	Norhazmiza Ahmad Safri, Rafidah Jalil, Mohd. Sahaid Kalil
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11662/ http://journalarticle.ukm.my/11662/ http://journalarticle.ukm.my/11662/1/6.pdf

id	ukm-11662
recordtype	eprints
spelling	ukm-116622018-05-18T00:42:35Z http://journalarticle.ukm.my/11662/ Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus Norhazmiza Ahmad Safri, Rafidah Jalil, Mohd. Sahaid Kalil, The viability of fermentation process is very much depends on the use of a cheap carbon source from lignocellulosic materials. It needs to hydrolyse into simple and readily metabolize carbohydrate using cellulase enzyme. Pycnoporus sanguineus has been able to produce cellulase enzyme with high enzyme activity that can convert lignocellulosic materials into fermentable sugar. P. sanguineus was culture using α-cellulose as carbon source for enzyme production via submerged fermentation (SMF) at different agitation speeds (100 and 150 rpm). Crude cellulase enzyme extracted from fermentation broth by centrifugation was used in hydrolysis of sawdust and sugarcane bagasse at different substrate concentrations (1-5% w/v). It was found that crude cellulase enzyme contain three main enzymes components i.e. endoglucanase, exoglucanase and xylanase with maximum activity of 1.55, 0.45 and 8.0 U/mL respectively that achieved after four days of cultivation at agitation speed of 150 rpm. The most suitable temperature and substrate concentration for sawdust and sugarcane bagasse hydrolysis by cellulase from P. sanguineus was at 30°C and 5% (w/v) where sugarcane bagasse produced maximum of 59.10 g/L fermentable sugar as compared to from sawdust (58.84 g/L). Fermentable sugar productivity was the highest (2.45 g/L/h) when sawdust was use compared to 0.50 g/L/h for sugarcane bagasse. Fermentable sugar produced from agrowastes using cellulase enzymes of P. sanguineus has highest potential as a feedstock for biofuels and biochemicals production. Penerbit Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11662/1/6.pdf Norhazmiza Ahmad Safri, and Rafidah Jalil, and Mohd. Sahaid Kalil, (2017) Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus. Jurnal Kejuruteraan, 29 (2). pp. 105-111. ISSN 0128-0198 http://www.ukm.my/jkukm/in-press-volume-292-2017/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The viability of fermentation process is very much depends on the use of a cheap carbon source from lignocellulosic materials. It needs to hydrolyse into simple and readily metabolize carbohydrate using cellulase enzyme. Pycnoporus sanguineus has been able to produce cellulase enzyme with high enzyme activity that can convert lignocellulosic materials into fermentable sugar. P. sanguineus was culture using α-cellulose as carbon source for enzyme production via submerged fermentation (SMF) at different agitation speeds (100 and 150 rpm). Crude cellulase enzyme extracted from fermentation broth by centrifugation was used in hydrolysis of sawdust and sugarcane bagasse at different substrate concentrations (1-5% w/v). It was found that crude cellulase enzyme contain three main enzymes components i.e. endoglucanase, exoglucanase and xylanase with maximum activity of 1.55, 0.45 and 8.0 U/mL respectively that achieved after four days of cultivation at agitation speed of 150 rpm. The most suitable temperature and substrate concentration for sawdust and sugarcane bagasse hydrolysis by cellulase from P. sanguineus was at 30°C and 5% (w/v) where sugarcane bagasse produced maximum of 59.10 g/L fermentable sugar as compared to from sawdust (58.84 g/L). Fermentable sugar productivity was the highest (2.45 g/L/h) when sawdust was use compared to 0.50 g/L/h for sugarcane bagasse. Fermentable sugar produced from agrowastes using cellulase enzymes of P. sanguineus has highest potential as a feedstock for biofuels and biochemicals production.
format	Article
author	Norhazmiza Ahmad Safri, Rafidah Jalil, Mohd. Sahaid Kalil,
spellingShingle	Norhazmiza Ahmad Safri, Rafidah Jalil, Mohd. Sahaid Kalil, Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
author_facet	Norhazmiza Ahmad Safri, Rafidah Jalil, Mohd. Sahaid Kalil,
author_sort	Norhazmiza Ahmad Safri,
title	Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
title_short	Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
title_full	Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
title_fullStr	Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
title_full_unstemmed	Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
title_sort	fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11662/ http://journalarticle.ukm.my/11662/ http://journalarticle.ukm.my/11662/1/6.pdf
first_indexed	2023-09-18T20:00:51Z
last_indexed	2023-09-18T20:00:51Z
_version_	1777406838365487104

Fermentable sugars from agrowastes using cellulase enzymes from local white rot fungi pycnoporus sanguineus

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