A non-invasive technique for sex determination of monomorphic birds
We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW that is preserved within the avian Z and W sex chromosomes. The s...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society of Applied Biology
2015
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| Online Access: | http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/1/44_4_04.pdf |
| Summary: | We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman
Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW
that is preserved within the avian Z and W sex chromosomes. The species under study were Stripe-throated Bulbul (Pycnonotus
finlaysoni), Olive-winged Bulbul (Pycnonotus plumosus), White-throated Kingfisher (Halcyon smyrnensis) and Collared
Scops Owl (Otus lettia). PCR amplification using P2/P8 primer pairs produced single band in male (ZZ) and two bands in
female (ZW) birds that ranged between 300-400 base pairs in length. The PCR products were confirmed by the preliminary
experiment of known sex of both species of polymorphic birds. Thus, this PCR-based test is fast, accurate and applicable in
these species. Therefore, it could be used for many conservation studies notably in threatened or rare species. |
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