A non-invasive technique for sex determination of monomorphic birds
We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW that is preserved within the avian Z and W sex chromosomes. The s...
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Malaysian Society of Applied Biology
2015
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| Online Access: | http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/1/44_4_04.pdf |
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ukm-117872018-06-29T07:56:56Z http://journalarticle.ukm.my/11787/ A non-invasive technique for sex determination of monomorphic birds Jessey Angat, Farida Zuraina Md Yusof, We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW that is preserved within the avian Z and W sex chromosomes. The species under study were Stripe-throated Bulbul (Pycnonotus finlaysoni), Olive-winged Bulbul (Pycnonotus plumosus), White-throated Kingfisher (Halcyon smyrnensis) and Collared Scops Owl (Otus lettia). PCR amplification using P2/P8 primer pairs produced single band in male (ZZ) and two bands in female (ZW) birds that ranged between 300-400 base pairs in length. The PCR products were confirmed by the preliminary experiment of known sex of both species of polymorphic birds. Thus, this PCR-based test is fast, accurate and applicable in these species. Therefore, it could be used for many conservation studies notably in threatened or rare species. Malaysian Society of Applied Biology 2015-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11787/1/44_4_04.pdf Jessey Angat, and Farida Zuraina Md Yusof, (2015) A non-invasive technique for sex determination of monomorphic birds. Malaysian Applied Biology, 44 (4). pp. 27-30. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=554&catid=59:current-view&Itemid=56 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Kebangasaan Malaysia |
| building |
UKM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman
Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW
that is preserved within the avian Z and W sex chromosomes. The species under study were Stripe-throated Bulbul (Pycnonotus
finlaysoni), Olive-winged Bulbul (Pycnonotus plumosus), White-throated Kingfisher (Halcyon smyrnensis) and Collared
Scops Owl (Otus lettia). PCR amplification using P2/P8 primer pairs produced single band in male (ZZ) and two bands in
female (ZW) birds that ranged between 300-400 base pairs in length. The PCR products were confirmed by the preliminary
experiment of known sex of both species of polymorphic birds. Thus, this PCR-based test is fast, accurate and applicable in
these species. Therefore, it could be used for many conservation studies notably in threatened or rare species. |
| format |
Article |
| author |
Jessey Angat, Farida Zuraina Md Yusof, |
| spellingShingle |
Jessey Angat, Farida Zuraina Md Yusof, A non-invasive technique for sex determination of monomorphic birds |
| author_facet |
Jessey Angat, Farida Zuraina Md Yusof, |
| author_sort |
Jessey Angat, |
| title |
A non-invasive technique for sex determination of monomorphic birds |
| title_short |
A non-invasive technique for sex determination of monomorphic birds |
| title_full |
A non-invasive technique for sex determination of monomorphic birds |
| title_fullStr |
A non-invasive technique for sex determination of monomorphic birds |
| title_full_unstemmed |
A non-invasive technique for sex determination of monomorphic birds |
| title_sort |
non-invasive technique for sex determination of monomorphic birds |
| publisher |
Malaysian Society of Applied Biology |
| publishDate |
2015 |
| url |
http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/ http://journalarticle.ukm.my/11787/1/44_4_04.pdf |
| first_indexed |
2023-09-18T20:01:08Z |
| last_indexed |
2023-09-18T20:01:08Z |
| _version_ |
1777406856490123264 |