Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model

Excessive expression and activity of 11β-hydroxysteroid dehydrogenase enzyme type 1 (11β-HSD1) enzyme in mature adipocyte leads to obesity and metabolic syndrome. Fructose in drinking water was proven to induce metabolic syndrome in male Wistar rat. Hence, the aim of the study was to assess the effe...

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Main Authors:	Norshalizah M, Zar, Chi T, Farihah Suhaimi
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11865/ http://journalarticle.ukm.my/11865/ http://journalarticle.ukm.my/11865/1/7_norshalizah_et_al_pdf_91243.pdf

id	ukm-11865
recordtype	eprints
spelling	ukm-118652018-07-10T04:25:42Z http://journalarticle.ukm.my/11865/ Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model Norshalizah M, Zar, Chi T Farihah Suhaimi, Excessive expression and activity of 11β-hydroxysteroid dehydrogenase enzyme type 1 (11β-HSD1) enzyme in mature adipocyte leads to obesity and metabolic syndrome. Fructose in drinking water was proven to induce metabolic syndrome in male Wistar rat. Hence, the aim of the study was to assess the effects of expression and activity of 11β-HSD1 enzyme in the liver in an established metabolic syndrome rat model induced by fructose drinking water. Twelve male Wistar rats were randomly divided into two groups: Control group, C (n=6) and Fructose drinking water 20%, F20 (n=6). The food and fluid intake were given as ad libitum for eight weeks. At the end of the experiment, the expression of 11β-HSD1 enzyme in the liver was measured by immunohistochemical staining method. The score was given according to the intensity of the staining of the granules in the hepatocyte cytoplasm which was measured using double-blinded method. Meanwhile, the activity of 11β-HSD1 enzyme in the liver was measured using ELISA technique. Following eight weeks of consumption of fructose drinking water, the F20 group showed an increased in both expression and activity of 11β-HSD1 in the liver. The obtained data clearly suggest that 11β-HSD1 enzymes in the liver may play a role in the development of metabolic syndrome and its complications in male Wistar rat. Pusat Perubatan Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11865/1/7_norshalizah_et_al_pdf_91243.pdf Norshalizah M, and Zar, Chi T and Farihah Suhaimi, (2017) Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model. Medicine & Health, 12 (1). pp. 56-65. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/12/1
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Excessive expression and activity of 11β-hydroxysteroid dehydrogenase enzyme type 1 (11β-HSD1) enzyme in mature adipocyte leads to obesity and metabolic syndrome. Fructose in drinking water was proven to induce metabolic syndrome in male Wistar rat. Hence, the aim of the study was to assess the effects of expression and activity of 11β-HSD1 enzyme in the liver in an established metabolic syndrome rat model induced by fructose drinking water. Twelve male Wistar rats were randomly divided into two groups: Control group, C (n=6) and Fructose drinking water 20%, F20 (n=6). The food and fluid intake were given as ad libitum for eight weeks. At the end of the experiment, the expression of 11β-HSD1 enzyme in the liver was measured by immunohistochemical staining method. The score was given according to the intensity of the staining of the granules in the hepatocyte cytoplasm which was measured using double-blinded method. Meanwhile, the activity of 11β-HSD1 enzyme in the liver was measured using ELISA technique. Following eight weeks of consumption of fructose drinking water, the F20 group showed an increased in both expression and activity of 11β-HSD1 in the liver. The obtained data clearly suggest that 11β-HSD1 enzymes in the liver may play a role in the development of metabolic syndrome and its complications in male Wistar rat.
format	Article
author	Norshalizah M, Zar, Chi T Farihah Suhaimi,
spellingShingle	Norshalizah M, Zar, Chi T Farihah Suhaimi, Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
author_facet	Norshalizah M, Zar, Chi T Farihah Suhaimi,
author_sort	Norshalizah M,
title	Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
title_short	Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
title_full	Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
title_fullStr	Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
title_full_unstemmed	Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
title_sort	effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11865/ http://journalarticle.ukm.my/11865/ http://journalarticle.ukm.my/11865/1/7_norshalizah_et_al_pdf_91243.pdf
first_indexed	2023-09-18T20:01:19Z
last_indexed	2023-09-18T20:01:19Z
_version_	1777406867741343744

Effects of 11β-hydroxysteroid dehydrogenase type 1 enzyme in the liver in fructose induced metabolic syndrome rat model

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