Optimization of illumina Truseq Targeted RNA expression (TREx) library quality

RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimiza...

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Main Authors:	Nadiah Abu, Yeap, Swee Keong, Noraini Nordin, Tan, Sheau Wei, Ho, Wan Yong, Noorjahan Banu Alitheen
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf

id	ukm-12009
recordtype	eprints
spelling	ukm-120092018-08-21T07:14:49Z http://journalarticle.ukm.my/12009/ Optimization of illumina Truseq Targeted RNA expression (TREx) library quality Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyAenriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA. Penerbit Universiti Kebangsaan Malaysia 2018-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf Nadiah Abu, and Yeap, Swee Keong and Noraini Nordin, and Tan, Sheau Wei and Ho, Wan Yong and Noorjahan Banu Alitheen, (2018) Optimization of illumina Truseq Targeted RNA expression (TREx) library quality. Sains Malaysiana, 47 (2). pp. 303-308. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num2_2018/contentsVol47num2_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyAenriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA.
format	Article
author	Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen,
spellingShingle	Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
author_facet	Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen,
author_sort	Nadiah Abu,
title	Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
title_short	Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
title_full	Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
title_fullStr	Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
title_full_unstemmed	Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
title_sort	optimization of illumina truseq targeted rna expression (trex) library quality
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf
first_indexed	2023-09-18T20:01:39Z
last_indexed	2023-09-18T20:01:39Z
_version_	1777406888481128448

Optimization of illumina Truseq Targeted RNA expression (TREx) library quality

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