Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penebit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf |
| Summary: | The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use. |
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