Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana

The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems a...

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Main Authors:	Gan, Khai Swan, Tan, Chon Seng, Rofina Yasmin Othman, Jennifer Ann Harikrishna
Format:	Article
Language:	English
Published:	Penebit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf

id	ukm-12031
recordtype	eprints
spelling	ukm-120312018-09-09T23:17:51Z http://journalarticle.ukm.my/12031/ Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use. Penebit Universiti Kebangsaan Malaysia 2018-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf Gan, Khai Swan and Tan, Chon Seng and Rofina Yasmin Othman, and Jennifer Ann Harikrishna, (2018) Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana. Sains Malaysiana, 47 (3). pp. 499-509. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil3_2018/KandunganJilid47Bil3_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.
format	Article
author	Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna,
spellingShingle	Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
author_facet	Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna,
author_sort	Gan, Khai Swan
title	Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
title_short	Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
title_full	Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
title_fullStr	Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
title_full_unstemmed	Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
title_sort	transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in nicotiana benthamiana
publisher	Penebit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf
first_indexed	2023-09-18T20:01:42Z
last_indexed	2023-09-18T20:01:42Z
_version_	1777406891729616896

Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana

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