Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity

Phytophthora is one of the most important plant pathogens of perennial crops in the tropics. This study characterised the pathogen(s) responsible for cocoa black pod rot, rubber pod rot and durian stem canker. Eighteen Phytophthora isolates were collected from several states in Malaysia, namely Paha...

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Main Authors:	M. Latifah, S. Kamaruzaman, M.A. Zainal Abidin, S.A. Nusaibah
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12079/ http://journalarticle.ukm.my/12079/ http://journalarticle.ukm.my/12079/1/06%20M.%20Latifah.pdf

id	ukm-12079
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spelling	ukm-120792018-09-22T10:28:48Z http://journalarticle.ukm.my/12079/ Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity M. Latifah, S. Kamaruzaman, M.A. Zainal Abidin, S.A. Nusaibah, Phytophthora is one of the most important plant pathogens of perennial crops in the tropics. This study characterised the pathogen(s) responsible for cocoa black pod rot, rubber pod rot and durian stem canker. Eighteen Phytophthora isolates were collected from several states in Malaysia, namely Pahang, Johor and Selangor. A total of 12 Phytophthora palmivora and six Phytophthora nicotianae isolates were isolated and identified based on the morphological and molecular characteristics. Internal transcribed spacer (ITS) sequences enabled Phytophthora identification to species level. Inoculation of 18 isolates on detached leaves and unripen cocoa pods successfully demonstrated the progressive development of lesions on its original host. Lesions were also observed in all wounded detached leaves of young durian (clone D24) and rubber (clone RRIM600) and unripen cocoa pods (clone BAL244) regardless of host and isolates. Phytophthora isolates from durian (DSCI) and rubber (R4A) used to inoculate cocoa pods exhibited lesion sizes of 11.6±0.75 and 9.6±0.64 mm, respectively. However, Phytophthora isolates from cocoa (CPR25) developed a longer length of lesion of 18.6±0.47 mm. On durian leaves, Phytophthora isolate from rubber (R4A) and cocoa (CPR25) recorded 11.73±1.04 and 5.22±0.57 mm length of lesion while Phytophthora isolates from durian (DSCB4) produced 13.13±1.29 mm lesion on its native host. Isolates from cocoa (CPR22) and durian (DSCE3) infected rubber leaves with 3.74±0.48 and 2.55±0.31 mm length of lesion, in comparison to rubber isolates (R1B) with 5.43±0.23 mm length of lesion. Differences in the length of lesion demonstrated higher level of virulence on the native host. Penerbit Universiti Kebangsaan Malaysia 2018-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12079/1/06%20M.%20Latifah.pdf M. Latifah, and S. Kamaruzaman, and M.A. Zainal Abidin, and S.A. Nusaibah, (2018) Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity. Sains Malaysiana, 47 (5). pp. 909-921. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num5_2018/contentsVol47num5_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Phytophthora is one of the most important plant pathogens of perennial crops in the tropics. This study characterised the pathogen(s) responsible for cocoa black pod rot, rubber pod rot and durian stem canker. Eighteen Phytophthora isolates were collected from several states in Malaysia, namely Pahang, Johor and Selangor. A total of 12 Phytophthora palmivora and six Phytophthora nicotianae isolates were isolated and identified based on the morphological and molecular characteristics. Internal transcribed spacer (ITS) sequences enabled Phytophthora identification to species level. Inoculation of 18 isolates on detached leaves and unripen cocoa pods successfully demonstrated the progressive development of lesions on its original host. Lesions were also observed in all wounded detached leaves of young durian (clone D24) and rubber (clone RRIM600) and unripen cocoa pods (clone BAL244) regardless of host and isolates. Phytophthora isolates from durian (DSCI) and rubber (R4A) used to inoculate cocoa pods exhibited lesion sizes of 11.6±0.75 and 9.6±0.64 mm, respectively. However, Phytophthora isolates from cocoa (CPR25) developed a longer length of lesion of 18.6±0.47 mm. On durian leaves, Phytophthora isolate from rubber (R4A) and cocoa (CPR25) recorded 11.73±1.04 and 5.22±0.57 mm length of lesion while Phytophthora isolates from durian (DSCB4) produced 13.13±1.29 mm lesion on its native host. Isolates from cocoa (CPR22) and durian (DSCE3) infected rubber leaves with 3.74±0.48 and 2.55±0.31 mm length of lesion, in comparison to rubber isolates (R1B) with 5.43±0.23 mm length of lesion. Differences in the length of lesion demonstrated higher level of virulence on the native host.
format	Article
author	M. Latifah, S. Kamaruzaman, M.A. Zainal Abidin, S.A. Nusaibah,
spellingShingle	M. Latifah, S. Kamaruzaman, M.A. Zainal Abidin, S.A. Nusaibah, Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
author_facet	M. Latifah, S. Kamaruzaman, M.A. Zainal Abidin, S.A. Nusaibah,
author_sort	M. Latifah,
title	Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
title_short	Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
title_full	Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
title_fullStr	Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
title_full_unstemmed	Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity
title_sort	identification of phytophthora spp. from perennial crops in malaysia, its pathogenicity and cross-pathogenicity
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12079/ http://journalarticle.ukm.my/12079/ http://journalarticle.ukm.my/12079/1/06%20M.%20Latifah.pdf
first_indexed	2023-09-18T20:01:48Z
last_indexed	2023-09-18T20:01:48Z
_version_	1777406898586255360

Identification of Phytophthora spp. from perennial crops in Malaysia, its pathogenicity and cross-pathogenicity

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