Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing

Dietary study provides understanding in predator-prey relationships, yet diet of tropical forest birds is poorly understood. In this study, a non-invasive method, next-generation sequencing (Illumina MiSeq platform) was used to identify prey in the faecal samples of the Rufous-winged Philentoma (Phi...

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Main Authors:	Mohammad Saiful Mansor, Shukor Md. Nor, Rosli Ramli
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12095/ http://journalarticle.ukm.my/12095/ http://journalarticle.ukm.my/12095/1/22%20Mohammad%20Saiful%20Mansor.pdf

id	ukm-12095
recordtype	eprints
spelling	ukm-120952018-09-22T10:37:33Z http://journalarticle.ukm.my/12095/ Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing Mohammad Saiful Mansor, Shukor Md. Nor, Rosli Ramli, Dietary study provides understanding in predator-prey relationships, yet diet of tropical forest birds is poorly understood. In this study, a non-invasive method, next-generation sequencing (Illumina MiSeq platform) was used to identify prey in the faecal samples of the Rufous-winged Philentoma (Philentoma pyrhoptera). Dietary samples were collected in lowland tropical forest of central Peninsular Malaysia. A general invertebrate primer pair was used for the first time to assess diet of tropical birds. The USEARCH was used to cluster the COI mtDNA sequences into Operational Taxonomic Unit (OTU). OTU sequences were aligned and queried through the GenBank or Biodiversity of Life Database (BOLD). We identified 26 distinct arthropod taxa from 31 OTUs. Of all OTUs, there was three that could be identified up to species level, 20 to genus level, three to family level and five could not assigned to any taxa (the BLAST hits were poor). All sequences were identified to class Insecta belonging to 18 families from four orders, where Lepidoptera representing major insect order consumed by study bird species. This non-invasive molecular approach provides a practical and rapid technique to understand of how energy flows across ecosystems. This technique could be very useful to screen for possible particular pest insects consumed by insectivores (e.g. birds and bats) in crop plantation. A comprehensive arthropod studies and local reference sequences need to be added to the database to improve the proportion of sequences that can be identified. Penerbit Universiti Kebangsaan Malaysia 2018-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12095/1/22%20Mohammad%20Saiful%20Mansor.pdf Mohammad Saiful Mansor, and Shukor Md. Nor, and Rosli Ramli, (2018) Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing. Sains Malaysiana, 47 (5). pp. 1045-1050. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num5_2018/contentsVol47num5_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Dietary study provides understanding in predator-prey relationships, yet diet of tropical forest birds is poorly understood. In this study, a non-invasive method, next-generation sequencing (Illumina MiSeq platform) was used to identify prey in the faecal samples of the Rufous-winged Philentoma (Philentoma pyrhoptera). Dietary samples were collected in lowland tropical forest of central Peninsular Malaysia. A general invertebrate primer pair was used for the first time to assess diet of tropical birds. The USEARCH was used to cluster the COI mtDNA sequences into Operational Taxonomic Unit (OTU). OTU sequences were aligned and queried through the GenBank or Biodiversity of Life Database (BOLD). We identified 26 distinct arthropod taxa from 31 OTUs. Of all OTUs, there was three that could be identified up to species level, 20 to genus level, three to family level and five could not assigned to any taxa (the BLAST hits were poor). All sequences were identified to class Insecta belonging to 18 families from four orders, where Lepidoptera representing major insect order consumed by study bird species. This non-invasive molecular approach provides a practical and rapid technique to understand of how energy flows across ecosystems. This technique could be very useful to screen for possible particular pest insects consumed by insectivores (e.g. birds and bats) in crop plantation. A comprehensive arthropod studies and local reference sequences need to be added to the database to improve the proportion of sequences that can be identified.
format	Article
author	Mohammad Saiful Mansor, Shukor Md. Nor, Rosli Ramli,
spellingShingle	Mohammad Saiful Mansor, Shukor Md. Nor, Rosli Ramli, Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
author_facet	Mohammad Saiful Mansor, Shukor Md. Nor, Rosli Ramli,
author_sort	Mohammad Saiful Mansor,
title	Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
title_short	Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
title_full	Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
title_fullStr	Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
title_full_unstemmed	Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
title_sort	assessing diet of the rufous-winged philentoma (philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12095/ http://journalarticle.ukm.my/12095/ http://journalarticle.ukm.my/12095/1/22%20Mohammad%20Saiful%20Mansor.pdf
first_indexed	2023-09-18T20:01:51Z
last_indexed	2023-09-18T20:01:51Z
_version_	1777406900942405632

Assessing diet of the Rufous-winged Philentoma (Philentoma pyrhoptera) in lowland tropical forest using next-generation sequencing

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