Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time

Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time

The role of heat shock protein in reproduction is widely known as a molecular chaperone in aiding and repairing protein formation when stress occurred. The present objectives were to evaluate the effect of different thawing temperature and time on the expression of HSP70 gene expression and the capa...

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Main Authors: Syarifah Faezah Syed Mohamad, Siti Fatimah Ibrahim, Nur Hilwan Ismail, Khairul Osman, Farah Hanan Fathihah Jaafar, Chew, Fang Nang, Farida Zuraina Mohd Yusof
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12113/
http://journalarticle.ukm.my/12113/
http://journalarticle.ukm.my/12113/1/04%20Syarifah%20Faezah%20Syed%20Mohamad.pdf
id ukm-12113
recordtype eprints
spelling ukm-121132018-09-28T21:57:59Z http://journalarticle.ukm.my/12113/ Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time Syarifah Faezah Syed Mohamad, Siti Fatimah Ibrahim, Nur Hilwan Ismail, Khairul Osman, Farah Hanan Fathihah Jaafar, Chew, Fang Nang Farida Zuraina Mohd Yusof, The role of heat shock protein in reproduction is widely known as a molecular chaperone in aiding and repairing protein formation when stress occurred. The present objectives were to evaluate the effect of different thawing temperature and time on the expression of HSP70 gene expression and the capacitation status in cryopreserved bovine spermatozoa. Briefly, fresh ejaculates were obtained from three different adult bulls. The semen then underwent a sperm washing technique known as Magnetic Activated Cell Sorting System (MACS) and later on, cryopreserved. The sperm- containing straws were then thawed at five different thawing temperatures and time post-cryostorage; 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s and 80°C for 5 s. The RNA was extracted from each of the sperm’s pellets and converted to cDNA prior to the qPCR process. Capacitation status was then determined by means of CTC assay. The results showed that after the process of amplification, there is a significant different of HSP70 gene expression in MACS process samples when the thawing process was performed at 37°C for 30 s, with p<0.05. Furthermore, the CTC assay also showed that thawing at the same temperature gave less capacitated spermatozoa with p<0.05. As a conclusion, MACS yield spermatozoa with a better expression of HSP70 gene and less capacitated spermatozoa when thawing was done at 37°C for 30 s. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12113/1/04%20Syarifah%20Faezah%20Syed%20Mohamad.pdf Syarifah Faezah Syed Mohamad, and Siti Fatimah Ibrahim, and Nur Hilwan Ismail, and Khairul Osman, and Farah Hanan Fathihah Jaafar, and Chew, Fang Nang and Farida Zuraina Mohd Yusof, (2018) Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time. Sains Malaysiana, 47 (6). pp. 1101-1108. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
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institution Universiti Kebangasaan Malaysia
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language English
description The role of heat shock protein in reproduction is widely known as a molecular chaperone in aiding and repairing protein formation when stress occurred. The present objectives were to evaluate the effect of different thawing temperature and time on the expression of HSP70 gene expression and the capacitation status in cryopreserved bovine spermatozoa. Briefly, fresh ejaculates were obtained from three different adult bulls. The semen then underwent a sperm washing technique known as Magnetic Activated Cell Sorting System (MACS) and later on, cryopreserved. The sperm- containing straws were then thawed at five different thawing temperatures and time post-cryostorage; 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s and 80°C for 5 s. The RNA was extracted from each of the sperm’s pellets and converted to cDNA prior to the qPCR process. Capacitation status was then determined by means of CTC assay. The results showed that after the process of amplification, there is a significant different of HSP70 gene expression in MACS process samples when the thawing process was performed at 37°C for 30 s, with p<0.05. Furthermore, the CTC assay also showed that thawing at the same temperature gave less capacitated spermatozoa with p<0.05. As a conclusion, MACS yield spermatozoa with a better expression of HSP70 gene and less capacitated spermatozoa when thawing was done at 37°C for 30 s.
format Article
author Syarifah Faezah Syed Mohamad,
Siti Fatimah Ibrahim,
Nur Hilwan Ismail,
Khairul Osman,
Farah Hanan Fathihah Jaafar,
Chew, Fang Nang
Farida Zuraina Mohd Yusof,
spellingShingle Syarifah Faezah Syed Mohamad,
Siti Fatimah Ibrahim,
Nur Hilwan Ismail,
Khairul Osman,
Farah Hanan Fathihah Jaafar,
Chew, Fang Nang
Farida Zuraina Mohd Yusof,
Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
author_facet Syarifah Faezah Syed Mohamad,
Siti Fatimah Ibrahim,
Nur Hilwan Ismail,
Khairul Osman,
Farah Hanan Fathihah Jaafar,
Chew, Fang Nang
Farida Zuraina Mohd Yusof,
author_sort Syarifah Faezah Syed Mohamad,
title Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
title_short Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
title_full Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
title_fullStr Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
title_full_unstemmed Quantification of HSP70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
title_sort quantification of hsp70 gene expression and determination of capacitation status of magnetically separated cryopreserved bovine spermatozoa at different thawing temperature and time
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12113/
http://journalarticle.ukm.my/12113/
http://journalarticle.ukm.my/12113/1/04%20Syarifah%20Faezah%20Syed%20Mohamad.pdf
first_indexed 2023-09-18T20:01:53Z
last_indexed 2023-09-18T20:01:53Z
_version_ 1777406903269195776

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