Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis

Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus com...

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Main Authors:	Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12171/ http://journalarticle.ukm.my/12171/ http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf

id	ukm-12171
recordtype	eprints
spelling	ukm-121712018-10-09T20:57:56Z http://journalarticle.ukm.my/12171/ Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani, Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Seven endonucleases of AluI, BsaJI, HaeIII, HindIII, HinfI, MboI and MboII were examined for the ability to digest cyt b amplicon from each species. Genomic DNA of pork (Sus scrofa domestica) were differentiated from fishes by comparing the digestion patterns produced by similar amplified region and enzymes used. In the present study, it was demonstrated that fishes and pork DNA genome were successfully differentiated using all endonucleases except for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA detection in fish products. Penerbit Universiti Kebangsaan Malaysia 2018-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf Safiyyah Shahimi, and Sahilah Abd. Mutalib, and Wan Sakeenah Wan Nazri, and Aminah Abdullah, and Norrakiah Abdullah Sani, (2018) Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis. Sains Malaysiana, 47 (7). pp. 1535-1540. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num7_2018/contentsVol47num7_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Seven endonucleases of AluI, BsaJI, HaeIII, HindIII, HinfI, MboI and MboII were examined for the ability to digest cyt b amplicon from each species. Genomic DNA of pork (Sus scrofa domestica) were differentiated from fishes by comparing the digestion patterns produced by similar amplified region and enzymes used. In the present study, it was demonstrated that fishes and pork DNA genome were successfully differentiated using all endonucleases except for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA detection in fish products.
format	Article
author	Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani,
spellingShingle	Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani, Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
author_facet	Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani,
author_sort	Safiyyah Shahimi,
title	Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_short	Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_full	Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_fullStr	Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_full_unstemmed	Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_sort	comparison of dna profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (pcr-rflp) analysis
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12171/ http://journalarticle.ukm.my/12171/ http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf
first_indexed	2023-09-18T20:02:01Z
last_indexed	2023-09-18T20:02:01Z
_version_	1777406911596986368

Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis

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