Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes

This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected...

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Main Authors:	Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee, Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12202/ http://journalarticle.ukm.my/12202/ http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf

id	ukm-12202
recordtype	eprints
spelling	ukm-122022018-10-16T13:29:20Z http://journalarticle.ukm.my/12202/ Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected to direct DNA extraction and PCR reaction. PCR products were analysed using gel electrophoresis and subjected to sequence analysis. Thirteen out of 105 (12.38%) samples were amplified for 16S rRNA with an expected amplicon size of 330 bp, while 50 out of 105 (47.62%) samples showed amplification using rpoβ primers, but were not of expected size. Of the 13 16S rRNA amplified samples, only 5 were identified as Leptospira in the gene sequence analysis and clustered under uncultured group via phylogenetic tree. This study showed the DNA-based approach using PCR and sequence analysis is able to detect the presence of Leptospira, although environmental samples may contain diverse microbial populations that may complicate the detection. Overall, the study suggested the importance of surveillance for Leptospira from environmental samples. Penerbit Universiti Kebangsaan Malaysia 2018-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf Nurul Farhana Zulkifli, and Steffi Julan Wan, and Vasantha Kumari Neela, and Chee, Hui Yee and Siti Norbaya Masri, and Mazen M. Jamil Al-Obaidi, and Mohd Nasir Mohd Desa, (2018) Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes. Sains Malaysiana, 47 (8). pp. 1795-1800. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected to direct DNA extraction and PCR reaction. PCR products were analysed using gel electrophoresis and subjected to sequence analysis. Thirteen out of 105 (12.38%) samples were amplified for 16S rRNA with an expected amplicon size of 330 bp, while 50 out of 105 (47.62%) samples showed amplification using rpoβ primers, but were not of expected size. Of the 13 16S rRNA amplified samples, only 5 were identified as Leptospira in the gene sequence analysis and clustered under uncultured group via phylogenetic tree. This study showed the DNA-based approach using PCR and sequence analysis is able to detect the presence of Leptospira, although environmental samples may contain diverse microbial populations that may complicate the detection. Overall, the study suggested the importance of surveillance for Leptospira from environmental samples.
format	Article
author	Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa,
spellingShingle	Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
author_facet	Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa,
author_sort	Nurul Farhana Zulkifli,
title	Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
title_short	Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
title_full	Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
title_fullStr	Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
title_full_unstemmed	Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
title_sort	detection of leptospira species in environmental samples by amplification of 16s rrna and rpoβ genes
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12202/ http://journalarticle.ukm.my/12202/ http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf
first_indexed	2023-09-18T20:02:05Z
last_indexed	2023-09-18T20:02:05Z
_version_	1777406915887759360

Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes

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