Dose response for Auricularia auricula-judae against acute gamma irradiation
Auricularia auricula-judae is a jelly and ear-like mushroom well known as part of traditional Chinese medicine and cuisine. Issues in mushroom cultivation are related to sustaining sufficient amount of strains with genetic variations for selection and obtaining quality strains for mother cultures....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12304/ http://journalarticle.ukm.my/12304/ http://journalarticle.ukm.my/12304/1/46_01_11.pdf |
| Summary: | Auricularia auricula-judae is a jelly and ear-like mushroom well known as part of traditional Chinese medicine and cuisine.
Issues in mushroom cultivation are related to sustaining sufficient amount of strains with genetic variations for selection and
obtaining quality strains for mother cultures. Strain selections by growers are hampered by the narrow genetic variations of
existing mushrooms. Induced mutations through gamma radiation was found to significantly generate genetic variations in
mushroom. This study aims to determine dose response of A. auricula-judae when irradiated with gamma radiation. Samples
grown on semi solid PDA (Potato Dextrose Agar) media were exposed to gamma radiation from Cs-137 source in Biobeam
GM8000. Doses for acute gamma irradiation ranged from 0 Gy, 0.1 kGy, 0.2 kGy, 0.3 kGy, 0.4 kGy, 0.5 kGy, 0.6 kGy, 0.7
kGy, 0.8 kGy, 0.9 kGy, 1.0 kGy, 1.5 kGy, 2.0 kGy and 4.0 kGy at dose rate 0.013 kGy/min. Visual observations and
diameter growth measurements of mycelia were observed 2 days’ interval for 8 days. Results revealed mycelia density and
growth performance decreases with increasing radiation doses. Other morphological characters of irradiated mycelia remained
the same with control. LD50 for A. auricula-judae was determined at 1.5 kGy. Findings in this study are important for
induced mutation studies of A. auricula-judae and benefiting to the mushroom industry. |
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