Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root
Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By transforming the full-length Avin_16040 gene into a h...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12332/ http://journalarticle.ukm.my/12332/ http://journalarticle.ukm.my/12332/1/46_02_07.pdf |
| Summary: | Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis
deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By
transforming the full-length Avin_16040 gene into a heterologous host Escherichia coli, the recombinant clones displayed
filamentous cell shapes in contrast to the rod-shape of wild type cells. Besides full-length gene insert, some E. coli clones
were detected to contain truncated Avin_16040 gene inserts but still shape-shifted to filamentous cells. Further analysis by
DNA sequencing revealed the shape-shifting E. coli clones contained 3’-end truncated Avin_16040 gene, while E. coli clones
containing the 5’-end truncated Avin_16040 gene remained rod-shaped. The cell surface topographies of A. vinelandii and E.
coli cells in the presence and absence of Avin_16040 gene and in association with rice root adherence were analysed using
atomic force microscopy. |
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