Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection

Most of commercial latex products originated from natural rubber produced by Hevea brasiliensis. Hevea latex contained of rubber particles that are linked with numerous allergens protein. The allergens protein associates with rubber particles can cause allergic reaction that range from contact and...

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Main Authors:	Nurul Faziha I., Shamsul Bahri A.R., Suhaizan L., Iffah Hazirah M.N., Ngadin A.A.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12381/ http://journalarticle.ukm.my/12381/ http://journalarticle.ukm.my/12381/1/46_03_29.pdf

id	ukm-12381
recordtype	eprints
spelling	ukm-123812018-11-30T22:46:02Z http://journalarticle.ukm.my/12381/ Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection Nurul Faziha I., Shamsul Bahri A.R., Suhaizan L., Iffah Hazirah M.N., Ngadin A.A., Most of commercial latex products originated from natural rubber produced by Hevea brasiliensis. Hevea latex contained of rubber particles that are linked with numerous allergens protein. The allergens protein associates with rubber particles can cause allergic reaction that range from contact and systemic urticarial to asthma, anaplyaxis and even death. Although allergen proteins are well known to be involved in rubber synthesis of H. brasiliensis, their presence in this species as well as other Hevea species is still need to be determined. For sustainability production of high demanding commercial latex, this study is conducted to investigate the protein profiling of two allergens on eight Hevea species obtained from the 1995 germplasm collection. Latex collected from eight Hevea species were prepared to detect allergens protein of Hevb1 and Hevb3 using SDS Polyacrylamide Gel (SDS-PAGE). Immunoblotting technique was used to evaluate compatibility of allergens protein to bind with monoclonal and polyclonal antisera. The results showed that both of Hevb1 and Hevb3 proteins were detected in eight Hevea species. However, Hevb1 and Hevb3 proteins showed different ability in binding the monoclonal and polyclonal antisera. Data on protein profiling of eight Hevea species constitute a potential source of good trait in future plant breeding or as a key to have a future rubber tree with less or allergen free latex. Penerbit Universiti Kebangsaan Malaysia 2017-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12381/1/46_03_29.pdf Nurul Faziha I., and Shamsul Bahri A.R., and Suhaizan L., and Iffah Hazirah M.N., and Ngadin A.A., (2017) Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection. Malaysian Applied Biology, 46 (3). pp. 239-245. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=674&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Most of commercial latex products originated from natural rubber produced by Hevea brasiliensis. Hevea latex contained of rubber particles that are linked with numerous allergens protein. The allergens protein associates with rubber particles can cause allergic reaction that range from contact and systemic urticarial to asthma, anaplyaxis and even death. Although allergen proteins are well known to be involved in rubber synthesis of H. brasiliensis, their presence in this species as well as other Hevea species is still need to be determined. For sustainability production of high demanding commercial latex, this study is conducted to investigate the protein profiling of two allergens on eight Hevea species obtained from the 1995 germplasm collection. Latex collected from eight Hevea species were prepared to detect allergens protein of Hevb1 and Hevb3 using SDS Polyacrylamide Gel (SDS-PAGE). Immunoblotting technique was used to evaluate compatibility of allergens protein to bind with monoclonal and polyclonal antisera. The results showed that both of Hevb1 and Hevb3 proteins were detected in eight Hevea species. However, Hevb1 and Hevb3 proteins showed different ability in binding the monoclonal and polyclonal antisera. Data on protein profiling of eight Hevea species constitute a potential source of good trait in future plant breeding or as a key to have a future rubber tree with less or allergen free latex.
format	Article
author	Nurul Faziha I., Shamsul Bahri A.R., Suhaizan L., Iffah Hazirah M.N., Ngadin A.A.,
spellingShingle	Nurul Faziha I., Shamsul Bahri A.R., Suhaizan L., Iffah Hazirah M.N., Ngadin A.A., Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
author_facet	Nurul Faziha I., Shamsul Bahri A.R., Suhaizan L., Iffah Hazirah M.N., Ngadin A.A.,
author_sort	Nurul Faziha I.,
title	Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
title_short	Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
title_full	Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
title_fullStr	Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
title_full_unstemmed	Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection
title_sort	protein profiling of hevb1 and hevb3 in hevea species obtained from the 1995 germplasm collection
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12381/ http://journalarticle.ukm.my/12381/ http://journalarticle.ukm.my/12381/1/46_03_29.pdf
first_indexed	2023-09-18T20:02:29Z
last_indexed	2023-09-18T20:02:29Z
_version_	1777406940778856448

Protein profiling of Hevb1 and Hevb3 in Hevea species obtained from the 1995 germplasm collection

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