Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines

The mangrove plant is known to produce secondary metabolite compounds, mainly isoprenoids. Polyisoprenoids (dolichol and polyprenol) are known to have antimicrobial, anticancer and antiviral activity. Therefore, this study aimed to determine the cytotoxic effects of polyisoprenoids from Rhizophora m...

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Main Authors:	Dini Permata Sari, Mohammad Basyuni, Hasibuan, Poppy Anjelisa Zaitun, Ridha Wati, Sumardi
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12383/ http://journalarticle.ukm.my/12383/ http://journalarticle.ukm.my/12383/1/02%20Dini%20Permata%20Sari.pdf

id	ukm-12383
recordtype	eprints
spelling	ukm-123832018-12-07T23:13:20Z http://journalarticle.ukm.my/12383/ Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines Dini Permata Sari, Mohammad Basyuni, Hasibuan, Poppy Anjelisa Zaitun Ridha Wati, Sumardi, The mangrove plant is known to produce secondary metabolite compounds, mainly isoprenoids. Polyisoprenoids (dolichol and polyprenol) are known to have antimicrobial, anticancer and antiviral activity. Therefore, this study aimed to determine the cytotoxic effects of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves by evaluating the induction of apoptosis and cell cycle arrest and the inhibition of the expression of Bcl-2 and cyclin D1 in WiDr colon cancer cells. Cell death was determined based on IC50 values in MTT assays. The induction of apoptosis and alterations in the cell cycle were observed by flow cytometry. The expression of Bcl-2 and cyclin D1 proteins, which play a role in apoptosis and cell cycle regulation, was observed by immunocytochemistry. The results showed that polyisoprenoids from R. mucronata and C. tagal leaves exhibited toxicity against the WiDr cell line, with IC50 values of 278 ± 5.77 and 276 ± 9.54 μg/mL, respectively. Polyisoprenoids from R. mucronata and C. tagal leaves significantly induced apoptosis and caused cell cycle arrest in G0/G1 phase, while also decreasing the expression of Bcl-2 and cyclin D1. Our results confirmed that polyisoprenoids from R. mucronata and C. tagal leaves have the potential to be developed as anticancer agents for colon cancer. Penerbit Universiti Kebangsaan Malaysia 2018-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12383/1/02%20Dini%20Permata%20Sari.pdf Dini Permata Sari, and Mohammad Basyuni, and Hasibuan, Poppy Anjelisa Zaitun and Ridha Wati, and Sumardi, (2018) Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines. Sains Malaysiana, 47 (9). pp. 1953-1959. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The mangrove plant is known to produce secondary metabolite compounds, mainly isoprenoids. Polyisoprenoids (dolichol and polyprenol) are known to have antimicrobial, anticancer and antiviral activity. Therefore, this study aimed to determine the cytotoxic effects of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves by evaluating the induction of apoptosis and cell cycle arrest and the inhibition of the expression of Bcl-2 and cyclin D1 in WiDr colon cancer cells. Cell death was determined based on IC50 values in MTT assays. The induction of apoptosis and alterations in the cell cycle were observed by flow cytometry. The expression of Bcl-2 and cyclin D1 proteins, which play a role in apoptosis and cell cycle regulation, was observed by immunocytochemistry. The results showed that polyisoprenoids from R. mucronata and C. tagal leaves exhibited toxicity against the WiDr cell line, with IC50 values of 278 ± 5.77 and 276 ± 9.54 μg/mL, respectively. Polyisoprenoids from R. mucronata and C. tagal leaves significantly induced apoptosis and caused cell cycle arrest in G0/G1 phase, while also decreasing the expression of Bcl-2 and cyclin D1. Our results confirmed that polyisoprenoids from R. mucronata and C. tagal leaves have the potential to be developed as anticancer agents for colon cancer.
format	Article
author	Dini Permata Sari, Mohammad Basyuni, Hasibuan, Poppy Anjelisa Zaitun Ridha Wati, Sumardi,
spellingShingle	Dini Permata Sari, Mohammad Basyuni, Hasibuan, Poppy Anjelisa Zaitun Ridha Wati, Sumardi, Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
author_facet	Dini Permata Sari, Mohammad Basyuni, Hasibuan, Poppy Anjelisa Zaitun Ridha Wati, Sumardi,
author_sort	Dini Permata Sari,
title	Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
title_short	Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
title_full	Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
title_fullStr	Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
title_full_unstemmed	Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines
title_sort	cytotoxic effect of polyisoprenoids from rhizophora mucronata and ceriops tagal leaves against widr colon cancer cell lines
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12383/ http://journalarticle.ukm.my/12383/ http://journalarticle.ukm.my/12383/1/02%20Dini%20Permata%20Sari.pdf
first_indexed	2023-09-18T20:02:29Z
last_indexed	2023-09-18T20:02:29Z
_version_	1777406941070360576

Cytotoxic effect of polyisoprenoids from Rhizophora mucronata and Ceriops tagal leaves against widr colon cancer cell lines

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