Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples

Microscopy-based technique has been widely used in the detection of Blastocystis sp. This study was conducted to compare the techniques used for screening of Blastocystis sp., namely in vitro cultivation of stool specimens in Jones’ medium (IVC) followed by Wheatley Trichrome staining and direct exa...

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Main Authors:	Samseh Abdullah Noradilah, Norhayati Moktar, Lee, Ii Li, Fatmah Md Salleh, Tengku Shahrul Anuar
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12400/ http://journalarticle.ukm.my/12400/ http://journalarticle.ukm.my/12400/1/18%20Samseh%20Abdullah%20Noradilah.pdf

id	ukm-12400
recordtype	eprints
spelling	ukm-124002018-12-07T23:32:26Z http://journalarticle.ukm.my/12400/ Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples Samseh Abdullah Noradilah, Norhayati Moktar, Lee, Ii Li Fatmah Md Salleh, Tengku Shahrul Anuar, Microscopy-based technique has been widely used in the detection of Blastocystis sp. This study was conducted to compare the techniques used for screening of Blastocystis sp., namely in vitro cultivation of stool specimens in Jones’ medium (IVC) followed by Wheatley Trichrome staining and direct examination of stool samples preserved with polyvinyl alcohol (PVA) followed by Wheatley Trichrome staining with single-round polymerase chain reaction (PCR) as the reference technique. The study was performed on 466 stool samples obtained from the aboriginal community in Pahang, Malaysia. IVC showed higher detection rate of Blastocystis sp. (35.6%) than PVA (20.0%). Single-round PCR detected Blastocystis sp. in 41.0% of the stool specimens. The sensitivity and specificity of PVA and IVC in comparison to the reference technique were 75.3% (95% CI: 65.2-83.6) and 68.5% (CI: 63.7-73.3) and 88.6% (CI: 82.7-93.0) and 86.3% (CI: 81.9-90.0), respectively. The agreement between the reference technique and PVA showed statistically significant fair agreement by Cohen Kappa statistics of (K=0.318, p<0.001), meanwhile statistically significant substantial agreement was observed between PCR and IVC by Cohen Kappa (K=0.727, p<0.001). Therefore, in vitro cultivation in Jones’ medium followed by Wheatley Trichrome staining of stool specimens should be used as a screening technique in the detection of Blastocystis sp. infections. Penerbit Universiti Kebangsaan Malaysia 2018-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12400/1/18%20Samseh%20Abdullah%20Noradilah.pdf Samseh Abdullah Noradilah, and Norhayati Moktar, and Lee, Ii Li and Fatmah Md Salleh, and Tengku Shahrul Anuar, (2018) Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples. Sains Malaysiana, 47 (9). pp. 2099-2104. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Microscopy-based technique has been widely used in the detection of Blastocystis sp. This study was conducted to compare the techniques used for screening of Blastocystis sp., namely in vitro cultivation of stool specimens in Jones’ medium (IVC) followed by Wheatley Trichrome staining and direct examination of stool samples preserved with polyvinyl alcohol (PVA) followed by Wheatley Trichrome staining with single-round polymerase chain reaction (PCR) as the reference technique. The study was performed on 466 stool samples obtained from the aboriginal community in Pahang, Malaysia. IVC showed higher detection rate of Blastocystis sp. (35.6%) than PVA (20.0%). Single-round PCR detected Blastocystis sp. in 41.0% of the stool specimens. The sensitivity and specificity of PVA and IVC in comparison to the reference technique were 75.3% (95% CI: 65.2-83.6) and 68.5% (CI: 63.7-73.3) and 88.6% (CI: 82.7-93.0) and 86.3% (CI: 81.9-90.0), respectively. The agreement between the reference technique and PVA showed statistically significant fair agreement by Cohen Kappa statistics of (K=0.318, p<0.001), meanwhile statistically significant substantial agreement was observed between PCR and IVC by Cohen Kappa (K=0.727, p<0.001). Therefore, in vitro cultivation in Jones’ medium followed by Wheatley Trichrome staining of stool specimens should be used as a screening technique in the detection of Blastocystis sp. infections.
format	Article
author	Samseh Abdullah Noradilah, Norhayati Moktar, Lee, Ii Li Fatmah Md Salleh, Tengku Shahrul Anuar,
spellingShingle	Samseh Abdullah Noradilah, Norhayati Moktar, Lee, Ii Li Fatmah Md Salleh, Tengku Shahrul Anuar, Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
author_facet	Samseh Abdullah Noradilah, Norhayati Moktar, Lee, Ii Li Fatmah Md Salleh, Tengku Shahrul Anuar,
author_sort	Samseh Abdullah Noradilah,
title	Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
title_short	Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
title_full	Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
title_fullStr	Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
title_full_unstemmed	Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples
title_sort	comparison of direct examination and in vitro culture for the detection of blastocystis sp. in orang asli stool samples
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12400/ http://journalarticle.ukm.my/12400/ http://journalarticle.ukm.my/12400/1/18%20Samseh%20Abdullah%20Noradilah.pdf
first_indexed	2023-09-18T20:02:31Z
last_indexed	2023-09-18T20:02:31Z
_version_	1777406943441190912

Comparison of direct examination and in vitro culture for the detection of blastocystis sp. in Orang Asli stool samples

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