Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells

Bacterial adhesion to host cells is the most important probiotic character. However, the adhesion of probiotic should not affect the viability of the host cells. In this study, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20 and Lactobacillus pentosus strain S1 were tested for...

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Main Authors:	Ida Muryany, Lian, Hing Hiang, Ina-Salwany, Ahmad Rohi Ghazali, Mohd Zamri-Saad, Nor Fadilah Rajab
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12508/ http://journalarticle.ukm.my/12508/ http://journalarticle.ukm.my/12508/1/15%20Ida%20Muryany.pdf

id	ukm-12508
recordtype	eprints
spelling	ukm-125082019-01-28T21:16:17Z http://journalarticle.ukm.my/12508/ Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells Ida Muryany, Lian, Hing Hiang Ina-Salwany, Ahmad Rohi Ghazali, Mohd Zamri-Saad, Nor Fadilah Rajab, Bacterial adhesion to host cells is the most important probiotic character. However, the adhesion of probiotic should not affect the viability of the host cells. In this study, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20 and Lactobacillus pentosus strain S1 were tested for their cytotoxic effects through MTT assay and their ability to adhere and colonize on HT-29 and CCD-18Co intestinal cells as detected microscopically using light microscopy and Scanning Electron Microscopy (SEM). No cytotoxicity effects were observed on both intestinal cells following 24 h treatment with all Lactobacillus strains. Additionally, all strains demonstrated strong adhesive activity where more than 100 bacteria adhered to both intestinal cells although differences in the adhesion scores observed among different strains. The adhesion as observed via SEM showed an autoagreggative pattern and adhered as clusters on the surface of both intestinal cells. In conclusion, all three Lactobacillus strains are non-cytotoxic to both cells with strong adhesion ability on intestinal cells and this study also proved that Malaysian fermented fish are good source of probiotic bacteria. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12508/1/15%20Ida%20Muryany.pdf Ida Muryany, and Lian, Hing Hiang and Ina-Salwany, and Ahmad Rohi Ghazali, and Mohd Zamri-Saad, and Nor Fadilah Rajab, (2018) Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells. Sains Malaysiana, 47 (10). pp. 2391-2399. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Bacterial adhesion to host cells is the most important probiotic character. However, the adhesion of probiotic should not affect the viability of the host cells. In this study, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20 and Lactobacillus pentosus strain S1 were tested for their cytotoxic effects through MTT assay and their ability to adhere and colonize on HT-29 and CCD-18Co intestinal cells as detected microscopically using light microscopy and Scanning Electron Microscopy (SEM). No cytotoxicity effects were observed on both intestinal cells following 24 h treatment with all Lactobacillus strains. Additionally, all strains demonstrated strong adhesive activity where more than 100 bacteria adhered to both intestinal cells although differences in the adhesion scores observed among different strains. The adhesion as observed via SEM showed an autoagreggative pattern and adhered as clusters on the surface of both intestinal cells. In conclusion, all three Lactobacillus strains are non-cytotoxic to both cells with strong adhesion ability on intestinal cells and this study also proved that Malaysian fermented fish are good source of probiotic bacteria.
format	Article
author	Ida Muryany, Lian, Hing Hiang Ina-Salwany, Ahmad Rohi Ghazali, Mohd Zamri-Saad, Nor Fadilah Rajab,
spellingShingle	Ida Muryany, Lian, Hing Hiang Ina-Salwany, Ahmad Rohi Ghazali, Mohd Zamri-Saad, Nor Fadilah Rajab, Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
author_facet	Ida Muryany, Lian, Hing Hiang Ina-Salwany, Ahmad Rohi Ghazali, Mohd Zamri-Saad, Nor Fadilah Rajab,
author_sort	Ida Muryany,
title	Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
title_short	Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
title_full	Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
title_fullStr	Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
title_full_unstemmed	Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells
title_sort	adhesion ability and cytotoxic evaluation of lactobacillus strains isolated from malaysian fermented fish (pekasam) on ht-29 and ccd-18co intestinal cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12508/ http://journalarticle.ukm.my/12508/ http://journalarticle.ukm.my/12508/1/15%20Ida%20Muryany.pdf
first_indexed	2023-09-18T20:02:46Z
last_indexed	2023-09-18T20:02:46Z
_version_	1777406958919221248

Adhesion ability and cytotoxic evaluation of Lactobacillus strains isolated from Malaysian fermented fish (Pekasam) on Ht-29 and Ccd-18Co intestinal cells

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