Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats
Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of thes...
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12522/ http://journalarticle.ukm.my/12522/ http://journalarticle.ukm.my/12522/1/28%20Helmy%20Mohd%20Mokhtar.pdf |
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ukm-125222019-01-28T21:38:20Z http://journalarticle.ukm.my/12522/ Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats Helmy Mohd Mokhtar, Nelli Giribabu, Naquib Salleh, Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of these molecules during early pregnancy. 30 ovariectomised female Sprague-Dawley rats were divided into 5 groups that consisted of vehicle control, rats received eight days sex-steroid replacement regime (intended to mimic the hormonal changes in early pregnancy) and three groups of rats given testosterone (1 mg/kg/day) subcutaneously with or without flutamide or finasteride between day 6 and 8 representing the period of uterine receptivity. At the end of the treatment, rats were sacrificed and uteri were removed. Expression and distribution of αvβ3 integrin, E-cadherin and mucin-1 were examined by immunoflourescence and levels of messenger RNA (mRNAs) were evaluated by real-time PCR. Expression of αvβ3 integrin, E-cadherin and mucin-1 in the uteri of rats receiving sex-steroid replacement regime increased significantly as compared to control (p<0.05). In these rats, concomitant administration of testosterone between day 6 and 8 resulted in expression of αvβ3 integrin, E-cadherin and mucin-1 to decrease significantly (p<0.05) as compared to rats receiving sex-steroid replacement regime without testosterone treatment. Moreover, the testosterone effects were not antagonized by either flutamide or finasteride. As a result, reduced expression of uterine receptivity molecules by testosterone might interfere with early pregnancy establishment, therefore could adversely affect the female fertility. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12522/1/28%20Helmy%20Mohd%20Mokhtar.pdf Helmy Mohd Mokhtar, and Nelli Giribabu, and Naquib Salleh, (2018) Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats. Sains Malaysiana, 47 (10). pp. 2509-2517. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm |
| repository_type |
Digital Repository |
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Local University |
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Universiti Kebangasaan Malaysia |
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UKM Institutional Repository |
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Online Access |
| language |
English |
| description |
Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of these molecules during early pregnancy. 30 ovariectomised female Sprague-Dawley rats were divided into 5 groups that consisted of vehicle control, rats received eight days sex-steroid replacement regime (intended to mimic the hormonal changes in early pregnancy) and three groups of rats given testosterone (1 mg/kg/day) subcutaneously with or without flutamide or finasteride between day 6 and 8 representing the period of uterine receptivity. At the end of the treatment, rats were sacrificed and uteri were removed. Expression and distribution of αvβ3 integrin, E-cadherin and mucin-1 were examined by immunoflourescence and levels of messenger RNA (mRNAs) were evaluated by real-time PCR. Expression of αvβ3 integrin, E-cadherin and mucin-1 in the uteri of rats receiving sex-steroid replacement regime increased significantly as compared to control (p<0.05). In these rats, concomitant administration of testosterone between day 6 and 8 resulted in expression of αvβ3 integrin, E-cadherin and mucin-1 to decrease significantly (p<0.05) as compared to rats receiving sex-steroid replacement regime without testosterone treatment. Moreover, the testosterone effects were not antagonized by either flutamide or finasteride. As a result, reduced expression of uterine receptivity molecules by testosterone might interfere with early pregnancy establishment, therefore could adversely affect the female fertility. |
| format |
Article |
| author |
Helmy Mohd Mokhtar, Nelli Giribabu, Naquib Salleh, |
| spellingShingle |
Helmy Mohd Mokhtar, Nelli Giribabu, Naquib Salleh, Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| author_facet |
Helmy Mohd Mokhtar, Nelli Giribabu, Naquib Salleh, |
| author_sort |
Helmy Mohd Mokhtar, |
| title |
Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| title_short |
Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| title_full |
Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| title_fullStr |
Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| title_full_unstemmed |
Testosterone down-regulates expression of αvβ3-integrin, E-cadherin and mucin-1 during uterine receptivity period in rats |
| title_sort |
testosterone down-regulates expression of αvβ3-integrin, e-cadherin and mucin-1 during uterine receptivity period in rats |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2018 |
| url |
http://journalarticle.ukm.my/12522/ http://journalarticle.ukm.my/12522/ http://journalarticle.ukm.my/12522/1/28%20Helmy%20Mohd%20Mokhtar.pdf |
| first_indexed |
2023-09-18T20:02:48Z |
| last_indexed |
2023-09-18T20:02:48Z |
| _version_ |
1777406960803512320 |