Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold

In vivo stability of biomaterial-based bone scaffolds often present a significant drawback in the development of materials for tissue engineering purpose. Previously developed nanobiocomposite bone scaffold using alginate and nano cockle shell powder has shown ideal characteristics. However, it show...

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Main Authors:	Hemabarathy Bharatham, Siti Fathiah Masre, Leo, Hwee Xien, Nurnadiah Ahmad
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12526/ http://journalarticle.ukm.my/12526/ http://journalarticle.ukm.my/12526/1/32%20Hemabarathy%20Bharatham.pdf

id	ukm-12526
recordtype	eprints
spelling	ukm-125262019-01-28T21:44:06Z http://journalarticle.ukm.my/12526/ Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold Hemabarathy Bharatham, Siti Fathiah Masre, Leo, Hwee Xien Nurnadiah Ahmad, In vivo stability of biomaterial-based bone scaffolds often present a significant drawback in the development of materials for tissue engineering purpose. Previously developed nanobiocomposite bone scaffold using alginate and nano cockle shell powder has shown ideal characteristics. However, it showed high degradation rate and reduced stability in an in vivo setting. In this study, we aim to observe the effect of cross-linking glutaraldehyde (GA) in three different concentrations of 0.5%, 1% and 2% during the fabrication process as a potential factor in increasing scaffold stability. Microstructure observations of scaffolds using scanning electron microscope (SEM) showed all scaffolds crossed linked with GA and control had an ideal pore size ranging from 166.8-203.5 μm. Increase in porosity compared to the control scaffolds was observed in scaffolds cross-linked with 2% GA which also presented better structural integrity as scored through semi-quantitative methods. Tested pH values during the degradation period showed that scaffolds from all groups remained within the range of 7.73-8.76. In vitro studies using osteoblast showed no significant changes in cell viability but a significant increase in ALP enzyme levels in scaffold cross-linked with 2% GA. The calcium content released from all scaffold showed significant differences within and between the groups. It can be concluded that the use of GA in the preparation stage of the scaffold did not affect the growth and proliferation of osteoblast and use of 2% GA showed improved scaffold structural integrity and porosity. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12526/1/32%20Hemabarathy%20Bharatham.pdf Hemabarathy Bharatham, and Siti Fathiah Masre, and Leo, Hwee Xien and Nurnadiah Ahmad, (2018) Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold. Sains Malaysiana, 47 (10). pp. 2557-2563. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In vivo stability of biomaterial-based bone scaffolds often present a significant drawback in the development of materials for tissue engineering purpose. Previously developed nanobiocomposite bone scaffold using alginate and nano cockle shell powder has shown ideal characteristics. However, it showed high degradation rate and reduced stability in an in vivo setting. In this study, we aim to observe the effect of cross-linking glutaraldehyde (GA) in three different concentrations of 0.5%, 1% and 2% during the fabrication process as a potential factor in increasing scaffold stability. Microstructure observations of scaffolds using scanning electron microscope (SEM) showed all scaffolds crossed linked with GA and control had an ideal pore size ranging from 166.8-203.5 μm. Increase in porosity compared to the control scaffolds was observed in scaffolds cross-linked with 2% GA which also presented better structural integrity as scored through semi-quantitative methods. Tested pH values during the degradation period showed that scaffolds from all groups remained within the range of 7.73-8.76. In vitro studies using osteoblast showed no significant changes in cell viability but a significant increase in ALP enzyme levels in scaffold cross-linked with 2% GA. The calcium content released from all scaffold showed significant differences within and between the groups. It can be concluded that the use of GA in the preparation stage of the scaffold did not affect the growth and proliferation of osteoblast and use of 2% GA showed improved scaffold structural integrity and porosity.
format	Article
author	Hemabarathy Bharatham, Siti Fathiah Masre, Leo, Hwee Xien Nurnadiah Ahmad,
spellingShingle	Hemabarathy Bharatham, Siti Fathiah Masre, Leo, Hwee Xien Nurnadiah Ahmad, Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
author_facet	Hemabarathy Bharatham, Siti Fathiah Masre, Leo, Hwee Xien Nurnadiah Ahmad,
author_sort	Hemabarathy Bharatham,
title	Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
title_short	Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
title_full	Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
title_fullStr	Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
title_full_unstemmed	Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold
title_sort	evaluating physical and biological characteristics of glutaraldehyde (ga) cross-linked nano-biocomposite bone scaffold
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12526/ http://journalarticle.ukm.my/12526/ http://journalarticle.ukm.my/12526/1/32%20Hemabarathy%20Bharatham.pdf
first_indexed	2023-09-18T20:02:48Z
last_indexed	2023-09-18T20:02:48Z
_version_	1777406961373937664

Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold

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