The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury

Centella asiatica (L.) Urban (CA) is a well- known plant used to improve brain and memory functions in traditional medicine. Scientifically it was proven to show neurogenic effect on neural cell lines and in rat’s hippocampus. Its effect on spinal cord (SC) neurons, however, have not been studied. A...

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Main Authors:	Nur Nabilah Ahmad Puzi, Yogeswaran Lokanathan, Ruszymah Hj. Idrus
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12660/ http://journalarticle.ukm.my/12660/ http://journalarticle.ukm.my/12660/1/21%20Nur%20Nabilah%20Ahmad%20Puzi.pdf

id	ukm-12660
recordtype	eprints
spelling	ukm-126602019-03-17T11:22:02Z http://journalarticle.ukm.my/12660/ The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury Nur Nabilah Ahmad Puzi, Yogeswaran Lokanathan, Ruszymah Hj. Idrus, Centella asiatica (L.) Urban (CA) is a well- known plant used to improve brain and memory functions in traditional medicine. Scientifically it was proven to show neurogenic effect on neural cell lines and in rat’s hippocampus. Its effect on spinal cord (SC) neurons, however, have not been studied. Aim of this study was to investigate the effects of raw extract of CA (RECA) on neurite outgrowths in an organotypic model of SC injury (OMSCI). OMSCI was prepared using SC slices obtained from postnatal-day 8 rat pups. Spinal cord tissues were embedded in gelatine gel and sliced to produce 300 μm thick slices. These slices were 100% viable for 8 days in culture. RECA, in concentrations of 0-800 μg/mL was added to the OMSCI media for 7 days, followed by immunostaining for TUJ-1 and GFAP. The investigated parameters were mean neurite count, mean neurite length, mean longest neurite and growth ratio. The tested RECA concentrations showed no cytotoxicity. ANOVA and Kruskal-Wallis tests showed no significant difference between groups in all the tested parameters. This may be due to low content of neurotrophic bioactive compounds content in the extract, which probably due to differences in geographical location, extraction method and absence of neurotrophic factors in the media. In conclusion, the tested RECA concentration were found to be safe; but without notable neurotrophic effects on the spinal cord organotypic model as demonstrated in this study. Penerbit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12660/1/21%20Nur%20Nabilah%20Ahmad%20Puzi.pdf Nur Nabilah Ahmad Puzi, and Yogeswaran Lokanathan, and Ruszymah Hj. Idrus, (2018) The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury. Sains Malaysiana, 47 (11). pp. 2783-2788. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num11_2018/contentsVol47num11_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Centella asiatica (L.) Urban (CA) is a well- known plant used to improve brain and memory functions in traditional medicine. Scientifically it was proven to show neurogenic effect on neural cell lines and in rat’s hippocampus. Its effect on spinal cord (SC) neurons, however, have not been studied. Aim of this study was to investigate the effects of raw extract of CA (RECA) on neurite outgrowths in an organotypic model of SC injury (OMSCI). OMSCI was prepared using SC slices obtained from postnatal-day 8 rat pups. Spinal cord tissues were embedded in gelatine gel and sliced to produce 300 μm thick slices. These slices were 100% viable for 8 days in culture. RECA, in concentrations of 0-800 μg/mL was added to the OMSCI media for 7 days, followed by immunostaining for TUJ-1 and GFAP. The investigated parameters were mean neurite count, mean neurite length, mean longest neurite and growth ratio. The tested RECA concentrations showed no cytotoxicity. ANOVA and Kruskal-Wallis tests showed no significant difference between groups in all the tested parameters. This may be due to low content of neurotrophic bioactive compounds content in the extract, which probably due to differences in geographical location, extraction method and absence of neurotrophic factors in the media. In conclusion, the tested RECA concentration were found to be safe; but without notable neurotrophic effects on the spinal cord organotypic model as demonstrated in this study.
format	Article
author	Nur Nabilah Ahmad Puzi, Yogeswaran Lokanathan, Ruszymah Hj. Idrus,
spellingShingle	Nur Nabilah Ahmad Puzi, Yogeswaran Lokanathan, Ruszymah Hj. Idrus, The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
author_facet	Nur Nabilah Ahmad Puzi, Yogeswaran Lokanathan, Ruszymah Hj. Idrus,
author_sort	Nur Nabilah Ahmad Puzi,
title	The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
title_short	The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
title_full	The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
title_fullStr	The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
title_full_unstemmed	The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury
title_sort	effect of centella asiatica (l.) urban on the organotypic model of spinal cord injury
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12660/ http://journalarticle.ukm.my/12660/ http://journalarticle.ukm.my/12660/1/21%20Nur%20Nabilah%20Ahmad%20Puzi.pdf
first_indexed	2023-09-18T20:03:06Z
last_indexed	2023-09-18T20:03:06Z
_version_	1777406980137156608

The effect of Centella asiatica (L.) Urban on the organotypic model of spinal cord injury

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