Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells

Development of resistance to currently used drugs and side effects of several allopathic drugs have led to increased emphasis on plant materials uses as a source of medicines for a wide variety of human illnesses including leukemia. Houttuynia cordata Thunb (H.cordata), a Northern Thailand local pla...

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Main Authors:	Boonyawee N, Chaowanee W, Paweena P, Supantitra Chanprasert
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12679/ http://journalarticle.ukm.my/12679/ http://journalarticle.ukm.my/12679/1/4_boonyawee_et_al_pdf_16392.pdf

id	ukm-12679
recordtype	eprints
spelling	ukm-126792019-03-17T12:04:59Z http://journalarticle.ukm.my/12679/ Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells Boonyawee N, Chaowanee W, Paweena P, Supantitra Chanprasert, Development of resistance to currently used drugs and side effects of several allopathic drugs have led to increased emphasis on plant materials uses as a source of medicines for a wide variety of human illnesses including leukemia. Houttuynia cordata Thunb (H.cordata), a Northern Thailand local plant and commonly known as Plucao, has various biological activities such as anti-inflammatory, anti-cancer and anti-leukemic activities. We aimed to determine active flavonoid components of H.cordata and to investigate the effect of H.cordata ethanolic extract on apoptotic induction on human acute lymphoblastic leukemia cells. In this study, we found that H.cordata ethanolic extract had total flavonoid of 231.21 ± 4.19 mg QE/g dried H.cordata. The extract analyzed by quantitative LC-MS consists of several flavonoid components including hyperin 6.35 ± 0.41, quercetin 0.34 ± 0.02, isoquercetin 1.10 ± 0.03, and rutin 0.88 ± 0.04 (%w/w). The cytotoxicity results showed dose dependent decrease in growth of Jurkat leukemic cells. Blebbing pattern of cell apoptosis was found in cells treated with H.cordata ethanolic extract for 24 and 48 hrs. Moreover, we found that the extract could substantially induce Jurkat cell death through apoptosis at both 12 and 24 hrs. In conclusion, these results indicated that H.cordata ethanolic extract which is composed of several flavonoids, possesses anti-leukemic activity through apoptotic induction in Jurkat leukemia cells. Pusat Perubatan Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12679/1/4_boonyawee_et_al_pdf_16392.pdf Boonyawee N, and Chaowanee W, and Paweena P, and Supantitra Chanprasert, (2017) Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells. Medicine & Health, 12 (2). pp. 193-201. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/12/2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Development of resistance to currently used drugs and side effects of several allopathic drugs have led to increased emphasis on plant materials uses as a source of medicines for a wide variety of human illnesses including leukemia. Houttuynia cordata Thunb (H.cordata), a Northern Thailand local plant and commonly known as Plucao, has various biological activities such as anti-inflammatory, anti-cancer and anti-leukemic activities. We aimed to determine active flavonoid components of H.cordata and to investigate the effect of H.cordata ethanolic extract on apoptotic induction on human acute lymphoblastic leukemia cells. In this study, we found that H.cordata ethanolic extract had total flavonoid of 231.21 ± 4.19 mg QE/g dried H.cordata. The extract analyzed by quantitative LC-MS consists of several flavonoid components including hyperin 6.35 ± 0.41, quercetin 0.34 ± 0.02, isoquercetin 1.10 ± 0.03, and rutin 0.88 ± 0.04 (%w/w). The cytotoxicity results showed dose dependent decrease in growth of Jurkat leukemic cells. Blebbing pattern of cell apoptosis was found in cells treated with H.cordata ethanolic extract for 24 and 48 hrs. Moreover, we found that the extract could substantially induce Jurkat cell death through apoptosis at both 12 and 24 hrs. In conclusion, these results indicated that H.cordata ethanolic extract which is composed of several flavonoids, possesses anti-leukemic activity through apoptotic induction in Jurkat leukemia cells.
format	Article
author	Boonyawee N, Chaowanee W, Paweena P, Supantitra Chanprasert,
spellingShingle	Boonyawee N, Chaowanee W, Paweena P, Supantitra Chanprasert, Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
author_facet	Boonyawee N, Chaowanee W, Paweena P, Supantitra Chanprasert,
author_sort	Boonyawee N,
title	Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
title_short	Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
title_full	Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
title_fullStr	Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
title_full_unstemmed	Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
title_sort	flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12679/ http://journalarticle.ukm.my/12679/ http://journalarticle.ukm.my/12679/1/4_boonyawee_et_al_pdf_16392.pdf
first_indexed	2023-09-18T20:03:09Z
last_indexed	2023-09-18T20:03:09Z
_version_	1777406982901202944

Flavonoid component determination and apoptotic induction evaluation of houttuynia cordata thunb extract on human acute leukemic cells

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