Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study

Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas...

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Main Authors:	Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12689/ http://journalarticle.ukm.my/12689/ http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf

id	ukm-12689
recordtype	eprints
spelling	ukm-126892019-03-17T12:16:50Z http://journalarticle.ukm.my/12689/ Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR, Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas and Methicillin-resistant Staphylococcus aureus (MRSA). In light of this information, a study was undertaken to detect these bacterial endosymbionts in Acanthamoeba spp. isolated from air-conditioning outlets in wards and operating theatres in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). The presence of these bacteria was screened using primer pair targeting each genus and further confirmed by sequencing analysis. Twenty-nine (80.56 %) Acanthamoeba isolates were found to contain targeted bacterial endosymbiont with at least one genus of bacteria per isolates. Mycobacterium spp. (82.76 %) were the most common bacteria detected, followed by Legionella spp. (65.52 %) and Pseudomonas spp. (62.07 %). No MRSA were detected in any isolates used in this study. Most of the Mycobacterium endosymbionts were non-tuberculous mycobacteria, while only two were part of Mycobacterium tuberculosis complex group. We conclude that most Acanthamoeba have the potential to host various pathogenic bacteria. However, the implication on the pathogenicity of both organisms remains unclear and further investigations are needed. Pusat Perubatan Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf Faizah Mohd Hanapiah, and Anisah Nordin, and Yusof Suboh, and Noraina AR, and Adibah MR, (2017) Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study. Medicine & Health, 12 (2). pp. 286-292. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/12/2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas and Methicillin-resistant Staphylococcus aureus (MRSA). In light of this information, a study was undertaken to detect these bacterial endosymbionts in Acanthamoeba spp. isolated from air-conditioning outlets in wards and operating theatres in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). The presence of these bacteria was screened using primer pair targeting each genus and further confirmed by sequencing analysis. Twenty-nine (80.56 %) Acanthamoeba isolates were found to contain targeted bacterial endosymbiont with at least one genus of bacteria per isolates. Mycobacterium spp. (82.76 %) were the most common bacteria detected, followed by Legionella spp. (65.52 %) and Pseudomonas spp. (62.07 %). No MRSA were detected in any isolates used in this study. Most of the Mycobacterium endosymbionts were non-tuberculous mycobacteria, while only two were part of Mycobacterium tuberculosis complex group. We conclude that most Acanthamoeba have the potential to host various pathogenic bacteria. However, the implication on the pathogenicity of both organisms remains unclear and further investigations are needed.
format	Article
author	Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR,
spellingShingle	Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR, Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
author_facet	Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR,
author_sort	Faizah Mohd Hanapiah,
title	Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_short	Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_full	Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_fullStr	Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_full_unstemmed	Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_sort	molecular detection of bacterial endosymbionts in acanthamoeba spp.: a preliminary study
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12689/ http://journalarticle.ukm.my/12689/ http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf
first_indexed	2023-09-18T20:03:10Z
last_indexed	2023-09-18T20:03:10Z
_version_	1777406984088190976

Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study

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