Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs

Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to form hyaline...

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Main Authors:	Alfaqeh, Hamoud Hussein, Ruszymah Haji Idrus, Chua, KH, Aminuddin S
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12690/ http://journalarticle.ukm.my/12690/ http://journalarticle.ukm.my/12690/1/13_hamoud_et_al_pdf_44993.pdf

id	ukm-12690
recordtype	eprints
spelling	ukm-126902019-03-17T12:19:22Z http://journalarticle.ukm.my/12690/ Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs Alfaqeh, Hamoud Hussein Ruszymah Haji Idrus, Chua, KH Aminuddin S, Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to form hyaline-like cartilage in vitro and in vivo when cultured in media supplemented with specific growth factors. This study aimed to examine the potential of BMSCs chondrogenesis in vitro and in vivo. BMSCs were isolated from sheep and divided into a test group and control group. Control group was cultured in medium supplemented with 10% fetal bovine serum (FBS). Test group was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL TGF-ß3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out on athymic nude mice. Cell aggregates were formed in test group and indicated the early chondrogenesis in cell culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 wks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group constructs showed higher cartilage matrix synthesis as confirmed by Safranin O staining and GAG production. These results demonstrated the effectiveness of low serum media supplemented with TGF-ß3 in promoting chondrogenesis in BMSCs. Pusat Perubatan Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12690/1/13_hamoud_et_al_pdf_44993.pdf Alfaqeh, Hamoud Hussein and Ruszymah Haji Idrus, and Chua, KH and Aminuddin S, (2017) Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs. Medicine & Health, 12 (2). pp. 293-303. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/12/2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to form hyaline-like cartilage in vitro and in vivo when cultured in media supplemented with specific growth factors. This study aimed to examine the potential of BMSCs chondrogenesis in vitro and in vivo. BMSCs were isolated from sheep and divided into a test group and control group. Control group was cultured in medium supplemented with 10% fetal bovine serum (FBS). Test group was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL TGF-ß3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out on athymic nude mice. Cell aggregates were formed in test group and indicated the early chondrogenesis in cell culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 wks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group constructs showed higher cartilage matrix synthesis as confirmed by Safranin O staining and GAG production. These results demonstrated the effectiveness of low serum media supplemented with TGF-ß3 in promoting chondrogenesis in BMSCs.
format	Article
author	Alfaqeh, Hamoud Hussein Ruszymah Haji Idrus, Chua, KH Aminuddin S,
spellingShingle	Alfaqeh, Hamoud Hussein Ruszymah Haji Idrus, Chua, KH Aminuddin S, Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
author_facet	Alfaqeh, Hamoud Hussein Ruszymah Haji Idrus, Chua, KH Aminuddin S,
author_sort	Alfaqeh, Hamoud Hussein
title	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_short	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_full	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_fullStr	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_full_unstemmed	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_sort	supplementation of tgf-beta3 in low serum media promotes chondrogenesis of bmscs
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12690/ http://journalarticle.ukm.my/12690/ http://journalarticle.ukm.my/12690/1/13_hamoud_et_al_pdf_44993.pdf
first_indexed	2023-09-18T20:03:10Z
last_indexed	2023-09-18T20:03:10Z
_version_	1777406984245477376

Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs

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