Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves

The leaves of Passiflora edulis (Passion fruit) traditionally used in American countries as a folk medicine to treat both anxiety and nervousness. They are rich in polyphenols which have been reported as natural antioxidants. In this study, five concentrations of ethanol (15%, 30%, 45%, 60% and 75...

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Main Authors:	Mohamad Khairi Zainol, Wong, Keat Yi, Zamzahaila Mohd Zin, Khairil Shazmin Kamarudin, Muhammad Danish-Daniel Abdullah, Ng, Khai Shin, Hasmadi Mamat
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12750/ http://journalarticle.ukm.my/12750/ http://journalarticle.ukm.my/12750/1/47_06_03.pdf

id	ukm-12750
recordtype	eprints
spelling	ukm-127502019-04-04T11:22:15Z http://journalarticle.ukm.my/12750/ Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves Mohamad Khairi Zainol, Wong, Keat Yi Zamzahaila Mohd Zin, Khairil Shazmin Kamarudin, Muhammad Danish-Daniel Abdullah, Ng, Khai Shin Hasmadi Mamat, The leaves of Passiflora edulis (Passion fruit) traditionally used in American countries as a folk medicine to treat both anxiety and nervousness. They are rich in polyphenols which have been reported as natural antioxidants. In this study, five concentrations of ethanol (15%, 30%, 45%, 60% and 75%) were used in the extraction of P. edulis leaves extracted using ultrasonic-assisted technique. Ultrasonic-assisted solvent extraction is a method that uses high frequency mechanical vibration to increase the effectiveness of the desire compound to be dissolved into the solvent. The total phenolic content and total flavonoid content were determined in this study. Antioxidant capacity was assessed with FTC, TBA and DPPH. The highest extraction yield was obtained using water extraction. The extract obtained by 75% ethanol showed the highest antioxidant activity by DPPH (2,2- diphenyl-1-picrylhydrazyl) radical scavenging assay (85.17 ± 0.36%) and Ferric Thiocyanate (FTC) test where the absorbance obtained was 0.09 ± 0.00. The same extract also exhibited the highest flavonoids content (90.94 ± 0.15 mg QE/g sample). The extract obtained by 15% ethanol exhibited the highest antioxidant activity in Thiobarbituric acid (TBA) assay (56.22 ± 5.01%). Apart from that, the highest phenolic content was found in 60% ethanol (57.08 ± 0.29 mg GAE/g sample). These results indicate that the leaves of P. edulis could be a potential source of natural antioxidants which can be used in dietary applications. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12750/1/47_06_03.pdf Mohamad Khairi Zainol, and Wong, Keat Yi and Zamzahaila Mohd Zin, and Khairil Shazmin Kamarudin, and Muhammad Danish-Daniel Abdullah, and Ng, Khai Shin and Hasmadi Mamat, (2018) Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves. Malaysian Applied Biology, 47 (6). pp. 19-27. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The leaves of Passiflora edulis (Passion fruit) traditionally used in American countries as a folk medicine to treat both anxiety and nervousness. They are rich in polyphenols which have been reported as natural antioxidants. In this study, five concentrations of ethanol (15%, 30%, 45%, 60% and 75%) were used in the extraction of P. edulis leaves extracted using ultrasonic-assisted technique. Ultrasonic-assisted solvent extraction is a method that uses high frequency mechanical vibration to increase the effectiveness of the desire compound to be dissolved into the solvent. The total phenolic content and total flavonoid content were determined in this study. Antioxidant capacity was assessed with FTC, TBA and DPPH. The highest extraction yield was obtained using water extraction. The extract obtained by 75% ethanol showed the highest antioxidant activity by DPPH (2,2- diphenyl-1-picrylhydrazyl) radical scavenging assay (85.17 ± 0.36%) and Ferric Thiocyanate (FTC) test where the absorbance obtained was 0.09 ± 0.00. The same extract also exhibited the highest flavonoids content (90.94 ± 0.15 mg QE/g sample). The extract obtained by 15% ethanol exhibited the highest antioxidant activity in Thiobarbituric acid (TBA) assay (56.22 ± 5.01%). Apart from that, the highest phenolic content was found in 60% ethanol (57.08 ± 0.29 mg GAE/g sample). These results indicate that the leaves of P. edulis could be a potential source of natural antioxidants which can be used in dietary applications.
format	Article
author	Mohamad Khairi Zainol, Wong, Keat Yi Zamzahaila Mohd Zin, Khairil Shazmin Kamarudin, Muhammad Danish-Daniel Abdullah, Ng, Khai Shin Hasmadi Mamat,
spellingShingle	Mohamad Khairi Zainol, Wong, Keat Yi Zamzahaila Mohd Zin, Khairil Shazmin Kamarudin, Muhammad Danish-Daniel Abdullah, Ng, Khai Shin Hasmadi Mamat, Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
author_facet	Mohamad Khairi Zainol, Wong, Keat Yi Zamzahaila Mohd Zin, Khairil Shazmin Kamarudin, Muhammad Danish-Daniel Abdullah, Ng, Khai Shin Hasmadi Mamat,
author_sort	Mohamad Khairi Zainol,
title	Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
title_short	Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
title_full	Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
title_fullStr	Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
title_full_unstemmed	Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves
title_sort	effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (passiflora edulis) leaves
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12750/ http://journalarticle.ukm.my/12750/ http://journalarticle.ukm.my/12750/1/47_06_03.pdf
first_indexed	2023-09-18T20:03:18Z
last_indexed	2023-09-18T20:03:18Z
_version_	1777406992565927936

Effect of ethanol in ultrasonic assisted extraction technique on antioxidative properties of passion fruit (Passiflora edulis) leaves

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