Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’

In vitro microrhizome induction is considered as an effective tool for high yielding rhizomatous crops. In this study, the effect of sucrose and BAP was examined to establish a suitable protocol for in vitro microrhizome production of Zingiber officinale Rosc. ‘Tambunan’. The in vitro derived plan...

Full description

Bibliographic Details
Main Authors:	Devina David, Ling, Ee Chuwan, Jualang Azlan Gansau
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12753/ http://journalarticle.ukm.my/12753/ http://journalarticle.ukm.my/12753/1/47_06_06.pdf

id	ukm-12753
recordtype	eprints
spelling	ukm-127532019-04-04T11:33:41Z http://journalarticle.ukm.my/12753/ Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’ Devina David, Ling, Ee Chuwan Jualang Azlan Gansau, In vitro microrhizome induction is considered as an effective tool for high yielding rhizomatous crops. In this study, the effect of sucrose and BAP was examined to establish a suitable protocol for in vitro microrhizome production of Zingiber officinale Rosc. ‘Tambunan’. The in vitro derived plantlets were used as explants and cultured on Murashige and Skoog (MS) medium treated with a combination of sucrose and BAP at various concentrations and maintained at 25 ± 2°C with 16 hr of photoperiod. After three months of culture, explant responded well on MS medium supplemented with 60 g/L of sucrose and 6 mg/L of BAP compared to other treatments. This treatment had significantly promoted the highest number of microrhizomes (seven) with a total weight of 2.90 g and a total number of 35 buds. Acclimatization of this microrhizome showed 88% of survivability rate after 21 days with a formation of new shoot and root. The current finding revealed the potential of microrhizomes for large-scale production of healthy planting material to support the ginger industry in this region. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12753/1/47_06_06.pdf Devina David, and Ling, Ee Chuwan and Jualang Azlan Gansau, (2018) Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’. Malaysian Applied Biology, 47 (6). pp. 47-52. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In vitro microrhizome induction is considered as an effective tool for high yielding rhizomatous crops. In this study, the effect of sucrose and BAP was examined to establish a suitable protocol for in vitro microrhizome production of Zingiber officinale Rosc. ‘Tambunan’. The in vitro derived plantlets were used as explants and cultured on Murashige and Skoog (MS) medium treated with a combination of sucrose and BAP at various concentrations and maintained at 25 ± 2°C with 16 hr of photoperiod. After three months of culture, explant responded well on MS medium supplemented with 60 g/L of sucrose and 6 mg/L of BAP compared to other treatments. This treatment had significantly promoted the highest number of microrhizomes (seven) with a total weight of 2.90 g and a total number of 35 buds. Acclimatization of this microrhizome showed 88% of survivability rate after 21 days with a formation of new shoot and root. The current finding revealed the potential of microrhizomes for large-scale production of healthy planting material to support the ginger industry in this region.
format	Article
author	Devina David, Ling, Ee Chuwan Jualang Azlan Gansau,
spellingShingle	Devina David, Ling, Ee Chuwan Jualang Azlan Gansau, Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
author_facet	Devina David, Ling, Ee Chuwan Jualang Azlan Gansau,
author_sort	Devina David,
title	Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
title_short	Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
title_full	Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
title_fullStr	Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
title_full_unstemmed	Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’
title_sort	optimizing sucrose and bap concentrations for in vitro microrhizome induction of zingiber officinale rosc. ‘tambunan’
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12753/ http://journalarticle.ukm.my/12753/ http://journalarticle.ukm.my/12753/1/47_06_06.pdf
first_indexed	2023-09-18T20:03:19Z
last_indexed	2023-09-18T20:03:19Z
_version_	1777406992995844096

Optimizing sucrose and BAP concentrations for in vitro microrhizome induction of Zingiber officinale Rosc. ‘Tambunan’

Similar Items