Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of thi...

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Main Authors:	Shaidatul Nadia Waludin, Ahmad Azfar Mohamed, Ing, Chia Phang, Yukinori Mukai
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12755/ http://journalarticle.ukm.my/12755/ http://journalarticle.ukm.my/12755/1/47_06_08.pdf

id	ukm-12755
recordtype	eprints
spelling	ukm-127552019-04-04T11:31:53Z http://journalarticle.ukm.my/12755/ Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data Shaidatul Nadia Waludin, Ahmad Azfar Mohamed, Ing, Chia Phang Yukinori Mukai, Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E. coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π) for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with 33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12755/1/47_06_08.pdf Shaidatul Nadia Waludin, and Ahmad Azfar Mohamed, and Ing, Chia Phang and Yukinori Mukai, (2018) Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data. Malaysian Applied Biology, 47 (6). pp. 61-68. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E. coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π) for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with 33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population.
format	Article
author	Shaidatul Nadia Waludin, Ahmad Azfar Mohamed, Ing, Chia Phang Yukinori Mukai,
spellingShingle	Shaidatul Nadia Waludin, Ahmad Azfar Mohamed, Ing, Chia Phang Yukinori Mukai, Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
author_facet	Shaidatul Nadia Waludin, Ahmad Azfar Mohamed, Ing, Chia Phang Yukinori Mukai,
author_sort	Shaidatul Nadia Waludin,
title	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_short	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_fullStr	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full_unstemmed	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_sort	genetic diversity of the orange-spotted grouper (epinephelus coioides) in terengganu malaysia based on mitochondrial cytochrome b sequence data
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12755/ http://journalarticle.ukm.my/12755/ http://journalarticle.ukm.my/12755/1/47_06_08.pdf
first_indexed	2023-09-18T20:03:19Z
last_indexed	2023-09-18T20:03:19Z
_version_	1777406993293639680

Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

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