Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis

Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis

Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclea...

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Main Authors: Akma Azmiera Akman, Ain Aisyah Talib, Azirah Md Yusof, Kamarul Ariffin Khalid, Nurul Asyiqin Yusof, Radiah Abdul Ghani, Asmah Hanim Hamdan, Zaitunnatakhin Zamli
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12761/
http://journalarticle.ukm.my/12761/
http://journalarticle.ukm.my/12761/1/47_06_11.pdf
id ukm-12761
recordtype eprints
spelling ukm-127612019-04-04T11:27:22Z http://journalarticle.ukm.my/12761/ Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis Akma Azmiera Akman, Ain Aisyah Talib, Azirah Md Yusof, Kamarul Ariffin Khalid, Nurul Asyiqin Yusof, Radiah Abdul Ghani, Asmah Hanim Hamdan, Zaitunnatakhin Zamli, Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the subchondral bone plate plays an important role in the pathogenesis of osteoarthritis. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12761/1/47_06_11.pdf Akma Azmiera Akman, and Ain Aisyah Talib, and Azirah Md Yusof, and Kamarul Ariffin Khalid, and Nurul Asyiqin Yusof, and Radiah Abdul Ghani, and Asmah Hanim Hamdan, and Zaitunnatakhin Zamli, (2018) Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis. Malaysian Applied Biology, 47 (6). pp. 87-96. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
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institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the subchondral bone plate plays an important role in the pathogenesis of osteoarthritis.
format Article
author Akma Azmiera Akman,
Ain Aisyah Talib,
Azirah Md Yusof,
Kamarul Ariffin Khalid,
Nurul Asyiqin Yusof,
Radiah Abdul Ghani,
Asmah Hanim Hamdan,
Zaitunnatakhin Zamli,
spellingShingle Akma Azmiera Akman,
Ain Aisyah Talib,
Azirah Md Yusof,
Kamarul Ariffin Khalid,
Nurul Asyiqin Yusof,
Radiah Abdul Ghani,
Asmah Hanim Hamdan,
Zaitunnatakhin Zamli,
Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
author_facet Akma Azmiera Akman,
Ain Aisyah Talib,
Azirah Md Yusof,
Kamarul Ariffin Khalid,
Nurul Asyiqin Yusof,
Radiah Abdul Ghani,
Asmah Hanim Hamdan,
Zaitunnatakhin Zamli,
author_sort Akma Azmiera Akman,
title Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_short Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_fullStr Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full_unstemmed Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_sort structural changes and the differential expression of osteoprotegerin (opg) and receptor activator of nuclear factor kb ligand (rankl) in subchondral bone during the development of osteoarthritis
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12761/
http://journalarticle.ukm.my/12761/
http://journalarticle.ukm.my/12761/1/47_06_11.pdf
first_indexed 2023-09-18T20:03:19Z
last_indexed 2023-09-18T20:03:19Z
_version_ 1777406993723555840

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