In vitro tolerance of rice pathogen Rhizoctonia solani by orchid mycorrhizal fungi isolated from orchids in BRIS, Setiu Wetlands
Rice (Oryza sativa) is a major staple food that feeds more than half of the human population in the world. However, rice production faces major loss in yield due to rice diseases including Rice Sheath Blight (RSB), which is caused by a soil-borne fungal pathogen, Rhizoctonia solani. Interestingly,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/12768/ http://journalarticle.ukm.my/12768/ http://journalarticle.ukm.my/12768/1/48_01_01.pdf |
| Summary: | Rice (Oryza sativa) is a major staple food that feeds more than half of the human population in the world. However, rice
production faces major loss in yield due to rice diseases including Rice Sheath Blight (RSB), which is caused by a soil-borne
fungal pathogen, Rhizoctonia solani. Interestingly, the same pathogenic R. solani in rice has also been isolated from orchids
and is known to form a vital symbiotic relationship, called orchid mycorrhiza. Thus, this study aims at isolating and identifying
the orchid mycorrhizal fungi (OMF) present in roots of orchids at beach ridges interspersed with swales (BRIS), Setiu
Wetlands and to determine their antagonistic activity towards pathogenic R. solani through dual culture bioassay. Mycorrhizal
fungi were isolated from the roots of Phalaenopsis pulcherrima, Bromheadia finlaysoniana and Dendrobium crumenatum.
Three isolates were identified through morphological identification to be Rhizoctonia species namely A, B and C along with
one fungal isolate as Daldinia species. In vitro antagonistic test against pathogenic R. solani showed Rhizoctonia species (B)
displayed the strongest anti-fungal activity with 60.46% of Percentage Inhibition of Radial Growth (PIRG) value followed
by Rhizoctonia species (C) with 59.9%, Rhizoctonia species (A) with 51.02% and Daldinia species with 39.80%. This
preliminary investigation shows the potential of orchid mycorrhizal fungi to be developed as a biocontrol agent. |
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