Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa

Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To e...

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Main Authors:	Kang, Wern Ni, Azila Adnan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12769/ http://journalarticle.ukm.my/12769/ http://journalarticle.ukm.my/12769/1/48_01_02.pdf

id	ukm-12769
recordtype	eprints
spelling	ukm-127692019-04-16T08:55:09Z http://journalarticle.ukm.my/12769/ Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa Kang, Wern Ni Azila Adnan, Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To enhance MC production, different combinations of the dualsaccharides, such as 25 g/L glucose + 25 g/L galactose, 25 g/L glucose + 25 g/L fructose, 25 g/L glucose + 25 g/L maltose and 25 g/L glucose + 25 g/L lactose were added into culture medium. The fermentation was carried out at 30oC for 5 days in incubator shaker. The combination of glucose and galactose (1:1) gave a higher concentration of MC with 1.23 g/L ± 0.15. However, it was lower than glucose (control) (1.8 g/L ± 0.63). The glucose + galactose concentrations varied between 10 g/ L, 30 g/L, 50 g/L and 70 g/L for the MC production. Therefore, the concentration of 50 g/L is the best for the the MC production. Increasing the concentration of glucose + galactose beyond 50 g/L decreased MC production. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12769/1/48_01_02.pdf Kang, Wern Ni and Azila Adnan, (2019) Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa. Malaysian Applied Biology, 48 (1). pp. 7-14. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To enhance MC production, different combinations of the dualsaccharides, such as 25 g/L glucose + 25 g/L galactose, 25 g/L glucose + 25 g/L fructose, 25 g/L glucose + 25 g/L maltose and 25 g/L glucose + 25 g/L lactose were added into culture medium. The fermentation was carried out at 30oC for 5 days in incubator shaker. The combination of glucose and galactose (1:1) gave a higher concentration of MC with 1.23 g/L ± 0.15. However, it was lower than glucose (control) (1.8 g/L ± 0.63). The glucose + galactose concentrations varied between 10 g/ L, 30 g/L, 50 g/L and 70 g/L for the MC production. Therefore, the concentration of 50 g/L is the best for the the MC production. Increasing the concentration of glucose + galactose beyond 50 g/L decreased MC production.
format	Article
author	Kang, Wern Ni Azila Adnan,
spellingShingle	Kang, Wern Ni Azila Adnan, Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
author_facet	Kang, Wern Ni Azila Adnan,
author_sort	Kang, Wern Ni
title	Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_short	Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_full	Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_fullStr	Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_full_unstemmed	Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_sort	effect of dual-saccharides on microbial cellulose (mc) production by pseudomonas aeruginosa
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12769/ http://journalarticle.ukm.my/12769/ http://journalarticle.ukm.my/12769/1/48_01_02.pdf
first_indexed	2023-09-18T20:03:20Z
last_indexed	2023-09-18T20:03:20Z
_version_	1777406994865455104

Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa

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