Study on genetic variability of mud crab, Scylla olivacea at east coast of Peninsular Malaysia using random amplified polymorphic DNA (RAPD): a preliminary assessment
Scylla olivacea is one of the mud crabs species and become source of economic income especially for local fishermen in Setiu Wetlands. Nevertheless, the genetic variability of this species are still poorly studied and need to be further clarified. Thus, the Random Amplified Polymorphic DNA-Polymer...
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/12770/ http://journalarticle.ukm.my/12770/ http://journalarticle.ukm.my/12770/1/48_01_03.pdf |
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ukm-127702019-04-16T09:04:55Z http://journalarticle.ukm.my/12770/ Study on genetic variability of mud crab, Scylla olivacea at east coast of Peninsular Malaysia using random amplified polymorphic DNA (RAPD): a preliminary assessment Wan Bayani Wan Omar, Faridah Mohamad, Ikhwanuddin @ Polity Abdullah, Mohd Firdaus Zainal Abidin, Scylla olivacea is one of the mud crabs species and become source of economic income especially for local fishermen in Setiu Wetlands. Nevertheless, the genetic variability of this species are still poorly studied and need to be further clarified. Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S. olivacea from Setiu Wetlands, Terengganu. The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. High polymorphism of S. olivacea revealed that this species are genetically variable. Further study should be done for a better understanding about variation and also for conservation and management of this species. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12770/1/48_01_03.pdf Wan Bayani Wan Omar, and Faridah Mohamad, and Ikhwanuddin @ Polity Abdullah, and Mohd Firdaus Zainal Abidin, (2019) Study on genetic variability of mud crab, Scylla olivacea at east coast of Peninsular Malaysia using random amplified polymorphic DNA (RAPD): a preliminary assessment. Malaysian Applied Biology, 48 (1). pp. 15-19. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Kebangasaan Malaysia |
| building |
UKM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
Scylla olivacea is one of the mud crabs species and become source of economic income especially for local fishermen in
Setiu Wetlands. Nevertheless, the genetic variability of this species are still poorly studied and need to be further clarified.
Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine
the genetic variability among individuals of S. olivacea from Setiu Wetlands, Terengganu. The genomic DNA was extracted
from ten samples of mud crab by using DNeasy kit. Twenty reproducible RAPD fragments generated by OPA 08 and OPA
10 with 17 fragments were polymorphic. The percentage of polymorphic bands was 85% indicates that the population of S.
olivacea has high level of polymorphism due to low inbreeding factor within a population. High polymorphism of S. olivacea
revealed that this species are genetically variable. Further study should be done for a better understanding about variation and
also for conservation and management of this species. |
| format |
Article |
| author |
Wan Bayani Wan Omar, Faridah Mohamad, Ikhwanuddin @ Polity Abdullah, Mohd Firdaus Zainal Abidin, |
| spellingShingle |
Wan Bayani Wan Omar, Faridah Mohamad, Ikhwanuddin @ Polity Abdullah, Mohd Firdaus Zainal Abidin, Study on genetic variability of mud crab, Scylla olivacea at east coast of Peninsular Malaysia using random amplified polymorphic DNA (RAPD): a preliminary assessment |
| author_facet |
Wan Bayani Wan Omar, Faridah Mohamad, Ikhwanuddin @ Polity Abdullah, Mohd Firdaus Zainal Abidin, |
| author_sort |
Wan Bayani Wan Omar, |
| title |
Study on genetic variability of mud crab,
Scylla olivacea at east coast of Peninsular Malaysia
using random amplified polymorphic DNA (RAPD):
a preliminary assessment |
| title_short |
Study on genetic variability of mud crab,
Scylla olivacea at east coast of Peninsular Malaysia
using random amplified polymorphic DNA (RAPD):
a preliminary assessment |
| title_full |
Study on genetic variability of mud crab,
Scylla olivacea at east coast of Peninsular Malaysia
using random amplified polymorphic DNA (RAPD):
a preliminary assessment |
| title_fullStr |
Study on genetic variability of mud crab,
Scylla olivacea at east coast of Peninsular Malaysia
using random amplified polymorphic DNA (RAPD):
a preliminary assessment |
| title_full_unstemmed |
Study on genetic variability of mud crab,
Scylla olivacea at east coast of Peninsular Malaysia
using random amplified polymorphic DNA (RAPD):
a preliminary assessment |
| title_sort |
study on genetic variability of mud crab,
scylla olivacea at east coast of peninsular malaysia
using random amplified polymorphic dna (rapd):
a preliminary assessment |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2019 |
| url |
http://journalarticle.ukm.my/12770/ http://journalarticle.ukm.my/12770/ http://journalarticle.ukm.my/12770/1/48_01_03.pdf |
| first_indexed |
2023-09-18T20:03:20Z |
| last_indexed |
2023-09-18T20:03:20Z |
| _version_ |
1777406995007012864 |