Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes

Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the effects of pineapple honey on the growth an...

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Main Authors:	Suhana Samat, Muhammad Ashraf Mohd Salleh, Zainah Adam, Wan Iryani Wan Ismail
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/1/48_01_04.pdf

id	ukm-12771
recordtype	eprints
spelling	ukm-127712019-04-16T09:02:51Z http://journalarticle.ukm.my/12771/ Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes Suhana Samat, Muhammad Ashraf Mohd Salleh, Zainah Adam, Wan Iryani Wan Ismail, Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the effects of pineapple honey on the growth and lipid accumulation of adipocytes in vitro. Pineapple honey was standardised according to its total phenolic and flavonoid contents prior to treating on differentiated 3T3-L1 adipocytes. Proliferation of adipocytes was observed using 3-(4,5 dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) assay while lipid accumulation and droplet size were determined using oil red O staining and Feret’s diameter. Pineapple honey exhibited 0.0379 ± 0.001 mg/100 mL GAE of total phenolic content and 0.098 ± 0.001 mg catechin/kg of total flavonoid content. It significantly inhibited adipocytes’ proliferation starting from 6.25% of pineapple honey concentration. In addition, the honey also significantly reduced lipid droplet size by 33.78% to 70.36% and reduced lipid accumulation compared to the control. These findings suggest that pineapple honey may affect the storage of lipids in adipocytes. Future investigation involving the biomarkers of adipogenesis is required to confirm whether the reduction in lipid accumulation is attributed to the effect of honey on these pathways. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12771/1/48_01_04.pdf Suhana Samat, and Muhammad Ashraf Mohd Salleh, and Zainah Adam, and Wan Iryani Wan Ismail, (2019) Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes. Malaysian Applied Biology, 48 (1). pp. 21-26. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the effects of pineapple honey on the growth and lipid accumulation of adipocytes in vitro. Pineapple honey was standardised according to its total phenolic and flavonoid contents prior to treating on differentiated 3T3-L1 adipocytes. Proliferation of adipocytes was observed using 3-(4,5 dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) assay while lipid accumulation and droplet size were determined using oil red O staining and Feret’s diameter. Pineapple honey exhibited 0.0379 ± 0.001 mg/100 mL GAE of total phenolic content and 0.098 ± 0.001 mg catechin/kg of total flavonoid content. It significantly inhibited adipocytes’ proliferation starting from 6.25% of pineapple honey concentration. In addition, the honey also significantly reduced lipid droplet size by 33.78% to 70.36% and reduced lipid accumulation compared to the control. These findings suggest that pineapple honey may affect the storage of lipids in adipocytes. Future investigation involving the biomarkers of adipogenesis is required to confirm whether the reduction in lipid accumulation is attributed to the effect of honey on these pathways.
format	Article
author	Suhana Samat, Muhammad Ashraf Mohd Salleh, Zainah Adam, Wan Iryani Wan Ismail,
spellingShingle	Suhana Samat, Muhammad Ashraf Mohd Salleh, Zainah Adam, Wan Iryani Wan Ismail, Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
author_facet	Suhana Samat, Muhammad Ashraf Mohd Salleh, Zainah Adam, Wan Iryani Wan Ismail,
author_sort	Suhana Samat,
title	Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
title_short	Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
title_full	Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
title_fullStr	Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
title_full_unstemmed	Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
title_sort	pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3t3-l1 adipocytes
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/1/48_01_04.pdf
first_indexed	2023-09-18T20:03:21Z
last_indexed	2023-09-18T20:03:21Z
_version_	1777406995149619200

Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes

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