Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+

This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 )...

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Main Authors:	Hazlina A.Z., Devanthiran L., Fatimah H.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12772/ http://journalarticle.ukm.my/12772/ http://journalarticle.ukm.my/12772/1/48_01_05.pdf

id	ukm-12772
recordtype	eprints
spelling	ukm-127722019-04-16T08:39:07Z http://journalarticle.ukm.my/12772/ Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+ Hazlina A.Z., Devanthiran L., Fatimah H., This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 ) of Hg2+ on the growth of C. vulgaris obtained from the dose-response curve was 0.72 mg/L. Under SEM, it was observed that Hg2+ treated cells become smaller in size (i.e. 2.1±0.1 μm) compared to normal cells (i.e. 3.2±0.1 μm). The morphology of cells changed from an intact microalgal cells with smooth spherical surface to a slightly roughened surface and shrivelled shape. Apoptotic bodies were also observed under 0.001, 0.01 and 0.1 mg/L of Hg2+ but not in 1.0 mg/L Hg2+. These results indicate that Hg2+ may induce apoptotic cell death at low concentrations but not at the highest concentration of 1.0 mg/L. The highest percentage of cells with comet level of 4 was observed in 1.0 mg/L Hg2+. However, Hg2+ is genotoxic to the microalga even at low concentration. In conclusion, Hg2+ can exert its toxic effects on C. vulgaris by changing the microalgal morphology, damaging the microalgal DNA and inducing cell death in the microalgal cells using different pathways at different concentrations. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12772/1/48_01_05.pdf Hazlina A.Z., and Devanthiran L., and Fatimah H., (2019) Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+. Malaysian Applied Biology, 48 (1). pp. 27-33. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 ) of Hg2+ on the growth of C. vulgaris obtained from the dose-response curve was 0.72 mg/L. Under SEM, it was observed that Hg2+ treated cells become smaller in size (i.e. 2.1±0.1 μm) compared to normal cells (i.e. 3.2±0.1 μm). The morphology of cells changed from an intact microalgal cells with smooth spherical surface to a slightly roughened surface and shrivelled shape. Apoptotic bodies were also observed under 0.001, 0.01 and 0.1 mg/L of Hg2+ but not in 1.0 mg/L Hg2+. These results indicate that Hg2+ may induce apoptotic cell death at low concentrations but not at the highest concentration of 1.0 mg/L. The highest percentage of cells with comet level of 4 was observed in 1.0 mg/L Hg2+. However, Hg2+ is genotoxic to the microalga even at low concentration. In conclusion, Hg2+ can exert its toxic effects on C. vulgaris by changing the microalgal morphology, damaging the microalgal DNA and inducing cell death in the microalgal cells using different pathways at different concentrations.
format	Article
author	Hazlina A.Z., Devanthiran L., Fatimah H.,
spellingShingle	Hazlina A.Z., Devanthiran L., Fatimah H., Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
author_facet	Hazlina A.Z., Devanthiran L., Fatimah H.,
author_sort	Hazlina A.Z.,
title	Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_short	Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_full	Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_fullStr	Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_full_unstemmed	Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_sort	morphological changes and dna damage in chlorella vulgaris (umt-m1) induced by hg2+
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12772/ http://journalarticle.ukm.my/12772/ http://journalarticle.ukm.my/12772/1/48_01_05.pdf
first_indexed	2023-09-18T20:03:21Z
last_indexed	2023-09-18T20:03:21Z
_version_	1777406995293274112

Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+

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