Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish

Malaysia is the 8th largest world producer of freshwater ornamental fish. Ornamental fish are commonly associated with Megalocytiviruses infection, which has led to severe diseases and economic loss. The purpose of this study was to detect the presence or absence of Infectious Spleen and Kidney Ne...

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Main Authors:	Sandra Catherine Zainathan, Dineswary Balaraman, Logajothiswaran Ambalavanan, Ponnarasi Krishna Moorthy, Surrin Kumar Palakrishnan, Nurshuhada Ariff
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12788/ http://journalarticle.ukm.my/12788/ http://journalarticle.ukm.my/12788/1/48_01_19.pdf

id	ukm-12788
recordtype	eprints
spelling	ukm-127882019-04-16T09:00:59Z http://journalarticle.ukm.my/12788/ Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish Sandra Catherine Zainathan, Dineswary Balaraman, Logajothiswaran Ambalavanan, Ponnarasi Krishna Moorthy, Surrin Kumar Palakrishnan, Nurshuhada Ariff, Malaysia is the 8th largest world producer of freshwater ornamental fish. Ornamental fish are commonly associated with Megalocytiviruses infection, which has led to severe diseases and economic loss. The purpose of this study was to detect the presence or absence of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in four ornamental fish species namely Xiphophorus maculatus, Poecilia reticulata, Trichogaster leeri and Apistogramma ramirezi from Malaysia. A total of 175 samples were analysed using PCR analysis for detection of ISKNV. The PCR analysis demonstrated 22 positive pooled samples (n = 110) for the presence of ISKNV. No clinical signs were observed in positive samples except darkened body in X. maculatus. Sequencing analysis of Megalocytivirus major capsid protein (MCP) revealed that the ISKNV strains in this study demonstrated high nucleotide identity to each other and reference ISKNV (96% to 100%). Based on the phylogenetic tree, the ISKNV strains were closely related to reference ISKNV and can be classified into Megalocytivirus genotype I. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12788/1/48_01_19.pdf Sandra Catherine Zainathan, and Dineswary Balaraman, and Logajothiswaran Ambalavanan, and Ponnarasi Krishna Moorthy, and Surrin Kumar Palakrishnan, and Nurshuhada Ariff, (2019) Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish. Malaysian Applied Biology, 48 (1). pp. 131-138. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Malaysia is the 8th largest world producer of freshwater ornamental fish. Ornamental fish are commonly associated with Megalocytiviruses infection, which has led to severe diseases and economic loss. The purpose of this study was to detect the presence or absence of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in four ornamental fish species namely Xiphophorus maculatus, Poecilia reticulata, Trichogaster leeri and Apistogramma ramirezi from Malaysia. A total of 175 samples were analysed using PCR analysis for detection of ISKNV. The PCR analysis demonstrated 22 positive pooled samples (n = 110) for the presence of ISKNV. No clinical signs were observed in positive samples except darkened body in X. maculatus. Sequencing analysis of Megalocytivirus major capsid protein (MCP) revealed that the ISKNV strains in this study demonstrated high nucleotide identity to each other and reference ISKNV (96% to 100%). Based on the phylogenetic tree, the ISKNV strains were closely related to reference ISKNV and can be classified into Megalocytivirus genotype I.
format	Article
author	Sandra Catherine Zainathan, Dineswary Balaraman, Logajothiswaran Ambalavanan, Ponnarasi Krishna Moorthy, Surrin Kumar Palakrishnan, Nurshuhada Ariff,
spellingShingle	Sandra Catherine Zainathan, Dineswary Balaraman, Logajothiswaran Ambalavanan, Ponnarasi Krishna Moorthy, Surrin Kumar Palakrishnan, Nurshuhada Ariff, Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
author_facet	Sandra Catherine Zainathan, Dineswary Balaraman, Logajothiswaran Ambalavanan, Ponnarasi Krishna Moorthy, Surrin Kumar Palakrishnan, Nurshuhada Ariff,
author_sort	Sandra Catherine Zainathan,
title	Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
title_short	Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
title_full	Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
title_fullStr	Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
title_full_unstemmed	Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish
title_sort	molecular screening of infectious spleen and kidney necrosis virus in four species of malaysian farmed ornamental fish
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12788/ http://journalarticle.ukm.my/12788/ http://journalarticle.ukm.my/12788/1/48_01_19.pdf
first_indexed	2023-09-18T20:03:23Z
last_indexed	2023-09-18T20:03:23Z
_version_	1777406997355823104

Molecular screening of infectious spleen and kidney necrosis virus in four species of Malaysian farmed ornamental fish

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