Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data

Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and...

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Main Authors:	Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf

id	ukm-12919
recordtype	eprints
spelling	ukm-129192019-05-15T11:00:48Z http://journalarticle.ukm.my/12919/ Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and reconstruct the secondary metabolite biosynthetic pathway of C. aeruginosa rhizome by integrating the metabolite profiling and transcriptomic data. A total of 81 metabolites were identified in the rhizome of C. aeruginosa; amongst others are curzerene and β-Cubebene which are potent antioxidants. A total of 28,225 unigene were obtained from the transcriptomic sequencing of C. aeruginosa rhizome and analysed to identify potential genes associated with the biosynthesis of its metabolites. Of these, 43 unigenes were identified and mapped onto five sub-pathways; i.e. carotenoid biosynthetic pathway, diterpenoid biosynthetic pathway, monoterpenoid biosynthetic pathway, terpenoid and steroid biosynthetic pathway, and sesquiterpenoid and triterpenoid biosynthetic pathway. This study demonstrated a systematic bioinformatic approach to reconstruct a metabolic pathway in the rhizome of C. aeruginosa using bioinformatic approach. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf Nurul-Syafika Mohamad-Fauzi, and Rabiatul-Adawiah Zainal-Abidin, and Mohd Waznul Adly Zaidan, and Sanimah Simoh, and Alizah Zainal, and Zeti-Azura Mohamed-Hussein, (2018) Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data. Sains Malaysiana, 47 (12). pp. 2985-2992. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and reconstruct the secondary metabolite biosynthetic pathway of C. aeruginosa rhizome by integrating the metabolite profiling and transcriptomic data. A total of 81 metabolites were identified in the rhizome of C. aeruginosa; amongst others are curzerene and β-Cubebene which are potent antioxidants. A total of 28,225 unigene were obtained from the transcriptomic sequencing of C. aeruginosa rhizome and analysed to identify potential genes associated with the biosynthesis of its metabolites. Of these, 43 unigenes were identified and mapped onto five sub-pathways; i.e. carotenoid biosynthetic pathway, diterpenoid biosynthetic pathway, monoterpenoid biosynthetic pathway, terpenoid and steroid biosynthetic pathway, and sesquiterpenoid and triterpenoid biosynthetic pathway. This study demonstrated a systematic bioinformatic approach to reconstruct a metabolic pathway in the rhizome of C. aeruginosa using bioinformatic approach.
format	Article
author	Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein,
spellingShingle	Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
author_facet	Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein,
author_sort	Nurul-Syafika Mohamad-Fauzi,
title	Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
title_short	Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
title_full	Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
title_fullStr	Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
title_full_unstemmed	Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
title_sort	reconstruction of curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf
first_indexed	2023-09-18T20:03:41Z
last_indexed	2023-09-18T20:03:41Z
_version_	1777407016565735424

Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data

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