Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan

Endophytic fungi are those living inside the host plant without causing any apparent negative effect on the host plant. Two isolates endophytic fungi from leaves and two isolates from root at Universiti Teknologi MARA (UiTM) Reserve Forest, Negeri Sembilan were successfully isolated and identified b...

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Main Authors:	Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor'Aishah Hasan, Nor'Aishah Abu Shah, Emelda Rosseleena Rohani
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12924/ http://journalarticle.ukm.my/12924/ http://journalarticle.ukm.my/12924/1/12%20Siti%20Nursyazwani%20Maadon.pdf

id	ukm-12924
recordtype	eprints
spelling	ukm-129242019-05-15T11:04:35Z http://journalarticle.ukm.my/12924/ Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor'Aishah Hasan, Nor'Aishah Abu Shah, Emelda Rosseleena Rohani, Endophytic fungi are those living inside the host plant without causing any apparent negative effect on the host plant. Two isolates endophytic fungi from leaves and two isolates from root at Universiti Teknologi MARA (UiTM) Reserve Forest, Negeri Sembilan were successfully isolated and identified by morphology and molecular characteristic. Samples were surface sterilized and sub-cultured to obtain a pure culture. Characteristics of the isolates such as colony appearance, mycelial texture, conidia/spores and pigmentation were studied to explore their morphology. Isolates were also subjected to a PCR-based genotyping test. There were noticeable differences in morphological characteristics among the four isolates. Microscopic analysis showed four isolates consist of septa and conidia/spores. The pigmentation result showed that colony in A1leaf samples demonstrated an orange color on potato dextrose agar (PDA) media, colony in A1root demonstrate a black texture in PDA media while hairy colonies in the others two isolates showed a white color on PDA media. Based on molecular analyses the fungal genera showed 99-100% similarity with the related fungi recorded in the GenBank. Both morphology and molecular sequencing of internal transcribed spacer (ITS) regions of endophytic fungi showed that three isolates (A1root, C2leaf, and C3root) were grouped in Basidiomycota while one isolate (A1leaf) belonged to Ascomycota. The endophyte funguses were identified as Daldinia sp. (A1leaf), Polyporales sp. (A1root,) Lentinus sp. (C2leaf,) and Rigidoporus sp. (C3root). Overall, the new discoveries of isolated endophyte fungal have dyeing potential of fungal pigments which offer a viable alternative to natural vegetable and harmful synthetic dyes. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12924/1/12%20Siti%20Nursyazwani%20Maadon.pdf Siti Nursyazwani Maadon, and Sarini Ahmad Wakid, and Iwana Izni Zainudin, and Lili Syahani Rusli, and Mohd Syahril Mohd Zan, and Nor'Aishah Hasan, and Nor'Aishah Abu Shah, and Emelda Rosseleena Rohani, (2018) Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan. Sains Malaysiana, 47 (12). pp. 3025-3030. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Endophytic fungi are those living inside the host plant without causing any apparent negative effect on the host plant. Two isolates endophytic fungi from leaves and two isolates from root at Universiti Teknologi MARA (UiTM) Reserve Forest, Negeri Sembilan were successfully isolated and identified by morphology and molecular characteristic. Samples were surface sterilized and sub-cultured to obtain a pure culture. Characteristics of the isolates such as colony appearance, mycelial texture, conidia/spores and pigmentation were studied to explore their morphology. Isolates were also subjected to a PCR-based genotyping test. There were noticeable differences in morphological characteristics among the four isolates. Microscopic analysis showed four isolates consist of septa and conidia/spores. The pigmentation result showed that colony in A1leaf samples demonstrated an orange color on potato dextrose agar (PDA) media, colony in A1root demonstrate a black texture in PDA media while hairy colonies in the others two isolates showed a white color on PDA media. Based on molecular analyses the fungal genera showed 99-100% similarity with the related fungi recorded in the GenBank. Both morphology and molecular sequencing of internal transcribed spacer (ITS) regions of endophytic fungi showed that three isolates (A1root, C2leaf, and C3root) were grouped in Basidiomycota while one isolate (A1leaf) belonged to Ascomycota. The endophyte funguses were identified as Daldinia sp. (A1leaf), Polyporales sp. (A1root,) Lentinus sp. (C2leaf,) and Rigidoporus sp. (C3root). Overall, the new discoveries of isolated endophyte fungal have dyeing potential of fungal pigments which offer a viable alternative to natural vegetable and harmful synthetic dyes.
format	Article
author	Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor'Aishah Hasan, Nor'Aishah Abu Shah, Emelda Rosseleena Rohani,
spellingShingle	Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor'Aishah Hasan, Nor'Aishah Abu Shah, Emelda Rosseleena Rohani, Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
author_facet	Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor'Aishah Hasan, Nor'Aishah Abu Shah, Emelda Rosseleena Rohani,
author_sort	Siti Nursyazwani Maadon,
title	Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
title_short	Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
title_full	Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
title_fullStr	Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
title_full_unstemmed	Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan
title_sort	isolation and identification of endophytic fungi from uitm reserve forest, negeri sembilan
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12924/ http://journalarticle.ukm.my/12924/ http://journalarticle.ukm.my/12924/1/12%20Siti%20Nursyazwani%20Maadon.pdf
first_indexed	2023-09-18T20:03:42Z
last_indexed	2023-09-18T20:03:42Z
_version_	1777407017288204288

Isolation and identification of endophytic fungi from UiTM Reserve Forest, Negeri Sembilan

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