Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS

Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either ch...

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Main Authors:	Lim, Choon Kiat, Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng, Kamalrul Azlan Azizan, Goh, You Keng, Goh, Kah Joo
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12928/ http://journalarticle.ukm.my/12928/ http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf

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spelling	ukm-129282019-05-15T11:07:55Z http://journalarticle.ukm.my/12928/ Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS Lim, Choon Kiat Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng Kamalrul Azlan Azizan, Goh, You Keng Goh, Kah Joo Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either chemical or cultural approaches, show varying degrees of effectiveness. The application of biological control agents which is environmental-friendly could be an attractive solution to overcome the problem. Earlier, we had isolated a mycoparasite, Scytalidium parasiticum, from the basidiomata of Ganoderma boninense. In vitro assay and nursery experiment showed that this fungus could suppress Ganoderma infection and reduce disease severity. However, metabolites which might contribute to the antagonistic or mycoparasitic effect remain unknown. In the current study, optimization of fungal sample processing, extraction, and analytical procedures were conducted to obtain metabolites from the maize substrate colonized by mycoparasitic ascomycetous Scytalidium parasiticum. This technique capable of producing sexual spores in sac-like organs. Untargeted metabolomics profiling was carried out by using Liquid Chromatography Time of Flight Mass Spectrometry (LC-ToF-MS). We found that S. parasiticum in both liquid- and solid-state cultivation gave higher metabolite when extracted with 60% methanol with 1% formic acid in combination with homogenisation methods such as ultrasonication and grinding. The findings from this study are useful for optimisation of metabolite extraction from other fungi-Ganoderma-plant interactions. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf Lim, Choon Kiat and Rafidah Ahmad, and Nurul Fadhilah Marzuki, and Goh, Yit Kheng and Kamalrul Azlan Azizan, and Goh, You Keng and Goh, Kah Joo (2018) Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS. Sains Malaysiana, 47 (12). pp. 3061-3068. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either chemical or cultural approaches, show varying degrees of effectiveness. The application of biological control agents which is environmental-friendly could be an attractive solution to overcome the problem. Earlier, we had isolated a mycoparasite, Scytalidium parasiticum, from the basidiomata of Ganoderma boninense. In vitro assay and nursery experiment showed that this fungus could suppress Ganoderma infection and reduce disease severity. However, metabolites which might contribute to the antagonistic or mycoparasitic effect remain unknown. In the current study, optimization of fungal sample processing, extraction, and analytical procedures were conducted to obtain metabolites from the maize substrate colonized by mycoparasitic ascomycetous Scytalidium parasiticum. This technique capable of producing sexual spores in sac-like organs. Untargeted metabolomics profiling was carried out by using Liquid Chromatography Time of Flight Mass Spectrometry (LC-ToF-MS). We found that S. parasiticum in both liquid- and solid-state cultivation gave higher metabolite when extracted with 60% methanol with 1% formic acid in combination with homogenisation methods such as ultrasonication and grinding. The findings from this study are useful for optimisation of metabolite extraction from other fungi-Ganoderma-plant interactions.
format	Article
author	Lim, Choon Kiat Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng Kamalrul Azlan Azizan, Goh, You Keng Goh, Kah Joo
spellingShingle	Lim, Choon Kiat Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng Kamalrul Azlan Azizan, Goh, You Keng Goh, Kah Joo Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
author_facet	Lim, Choon Kiat Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng Kamalrul Azlan Azizan, Goh, You Keng Goh, Kah Joo
author_sort	Lim, Choon Kiat
title	Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_short	Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_full	Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_fullStr	Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_full_unstemmed	Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_sort	optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic scytalidium parasiticum using lc-tof-ms
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12928/ http://journalarticle.ukm.my/12928/ http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf
first_indexed	2023-09-18T20:03:42Z
last_indexed	2023-09-18T20:03:42Z
_version_	1777407017868066816

Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS

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