Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus

There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites pro...

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Main Authors:	Mohd Azhar Hassan, Mariatulqabtiah Abdul Razak, Ahmad Hafiz Baharom, Muhammad Shafie Md Sah, Mohamad Zulkiffely A. Rahman
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12931/ http://journalarticle.ukm.my/12931/ http://journalarticle.ukm.my/12931/1/19%20Mohd%20Azhar%20Hassan.pdf

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recordtype	eprints
spelling	ukm-129312019-05-15T11:09:58Z http://journalarticle.ukm.my/12931/ Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus Mohd Azhar Hassan, Mariatulqabtiah Abdul Razak, Ahmad Hafiz Baharom, Muhammad Shafie Md Sah, Mohamad Zulkiffely A. Rahman, There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites production in plant. This study was conducted to determine the optimal conditions for P. minus root formation in vitro and to profile the metabolite content from P. minus root culture with and without elicitor treatment. From the root induction study, it was found that the fresh weight of induced root for nodal explant in MS liquid media supplemented with 0.5 mg/L NAA and shaken had the highest production (0.38±0.08 g) compared to other treatments including the control. The results from metabolite profile showed that the volatile compound of P. minus root produced without any elicitation contained 50.11% aliphatic (27.59% aldehide, 9.17% alkane and 13.35% others) and 19.39% sesquiterpene (β-caryophyllene, α-bergamotene, β-farnesene, α-caryophyllene dan β-curcumene) where the dodecanal compound (22.27%) and β-caryophyllene (8.09%) have the highest percentage value for aliphatic and sesquiterpene group, respectively. Moreover, elicitation of P. minus root culture using yeast extract at 100 mg/L concentration for 1 day demonstrated the ability to increase the production of secondary metabolites in many volatile compounds of kesum in vitro root including the sesquiterpene compounds compared to control treatment and other yeast extract elicitation treatments. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12931/1/19%20Mohd%20Azhar%20Hassan.pdf Mohd Azhar Hassan, and Mariatulqabtiah Abdul Razak, and Ahmad Hafiz Baharom, and Muhammad Shafie Md Sah, and Mohamad Zulkiffely A. Rahman, (2018) Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus. Sains Malaysiana, 47 (12). pp. 3085-3094. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites production in plant. This study was conducted to determine the optimal conditions for P. minus root formation in vitro and to profile the metabolite content from P. minus root culture with and without elicitor treatment. From the root induction study, it was found that the fresh weight of induced root for nodal explant in MS liquid media supplemented with 0.5 mg/L NAA and shaken had the highest production (0.38±0.08 g) compared to other treatments including the control. The results from metabolite profile showed that the volatile compound of P. minus root produced without any elicitation contained 50.11% aliphatic (27.59% aldehide, 9.17% alkane and 13.35% others) and 19.39% sesquiterpene (β-caryophyllene, α-bergamotene, β-farnesene, α-caryophyllene dan β-curcumene) where the dodecanal compound (22.27%) and β-caryophyllene (8.09%) have the highest percentage value for aliphatic and sesquiterpene group, respectively. Moreover, elicitation of P. minus root culture using yeast extract at 100 mg/L concentration for 1 day demonstrated the ability to increase the production of secondary metabolites in many volatile compounds of kesum in vitro root including the sesquiterpene compounds compared to control treatment and other yeast extract elicitation treatments.
format	Article
author	Mohd Azhar Hassan, Mariatulqabtiah Abdul Razak, Ahmad Hafiz Baharom, Muhammad Shafie Md Sah, Mohamad Zulkiffely A. Rahman,
spellingShingle	Mohd Azhar Hassan, Mariatulqabtiah Abdul Razak, Ahmad Hafiz Baharom, Muhammad Shafie Md Sah, Mohamad Zulkiffely A. Rahman, Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
author_facet	Mohd Azhar Hassan, Mariatulqabtiah Abdul Razak, Ahmad Hafiz Baharom, Muhammad Shafie Md Sah, Mohamad Zulkiffely A. Rahman,
author_sort	Mohd Azhar Hassan,
title	Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_short	Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_full	Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_fullStr	Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_full_unstemmed	Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_sort	effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of polygonum minus
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12931/ http://journalarticle.ukm.my/12931/ http://journalarticle.ukm.my/12931/1/19%20Mohd%20Azhar%20Hassan.pdf
first_indexed	2023-09-18T20:03:43Z
last_indexed	2023-09-18T20:03:43Z
_version_	1777407018300080128

Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus

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