Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions

Ficus deltoidea or ‘mas cotek,’ is a uterotonic herb traditionally consumed by women to improve menstrual circulation, assist labour, remove retained placenta and treat postpartum bleeding. The aim of the study was to elucidate the mechanism of F. deltoidea in uterine contraction. Crude extracts fro...

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Main Authors:	Farah Wahida Ibrahim, Nor Idayu Derased, Umi Romaizatul Amiera Zainudin, Nor Fadilah Rajab
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12943/ http://journalarticle.ukm.my/12943/ http://journalarticle.ukm.my/12943/1/24032-76983-1-PB.pdf

id	ukm-12943
recordtype	eprints
spelling	ukm-129432019-05-17T14:43:52Z http://journalarticle.ukm.my/12943/ Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions Farah Wahida Ibrahim, Nor Idayu Derased, Umi Romaizatul Amiera Zainudin, Nor Fadilah Rajab, Ficus deltoidea or ‘mas cotek,’ is a uterotonic herb traditionally consumed by women to improve menstrual circulation, assist labour, remove retained placenta and treat postpartum bleeding. The aim of the study was to elucidate the mechanism of F. deltoidea in uterine contraction. Crude extracts from 2 different variants of F. deltoidea were used in the study; F. deltoidea var. Deltoidea (FDD) and F. deltoidea var. Angustifolia (FDA). This study was conducted ex vivo on the strips of isolated rats uterus treated with either FDD or FDA aqueous extract with increasing concentrations ranging from 10 μg/ml until 1280 μg/ml at time intervals of 5 minutes between doses. The frequency and intensity of the uterine contractions were monitored via Powerlab software. Maximum contractions for both extracts were identified, recorded and the uterine strips samples at maximum contraction were selected and homogenized in order determine the role of prostaglandin F2α (PGF2α) in the mechanism of uterine contraction. Other than that, phosphorylated 42/44 (p42/44) of mitogen activated protein kinase (MAPK) expression was also detected via immunoblotting. The results showed that the maximum contraction induced by FDD was at the concentration of 320 μg/ml, whereas for FDA was at 960 μg/ml. Both FDD and FDA increased the intensity of uterine strips contractions and there were notable trend of increased PGF2α expression as well. Further analysis revealed that the uterine contractions involved the MAPK pathway through the phosphorylation of p42/44 protein. In conclusion, Ficus deltoidea of both variants have the ability to stimulate uterine contraction through the mechanism of MAPK pathway. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12943/1/24032-76983-1-PB.pdf Farah Wahida Ibrahim, and Nor Idayu Derased, and Umi Romaizatul Amiera Zainudin, and Nor Fadilah Rajab, (2018) Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions. Jurnal Sains Kesihatan Malaysia, 16 (SI). pp. 75-81. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1072
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Ficus deltoidea or ‘mas cotek,’ is a uterotonic herb traditionally consumed by women to improve menstrual circulation, assist labour, remove retained placenta and treat postpartum bleeding. The aim of the study was to elucidate the mechanism of F. deltoidea in uterine contraction. Crude extracts from 2 different variants of F. deltoidea were used in the study; F. deltoidea var. Deltoidea (FDD) and F. deltoidea var. Angustifolia (FDA). This study was conducted ex vivo on the strips of isolated rats uterus treated with either FDD or FDA aqueous extract with increasing concentrations ranging from 10 μg/ml until 1280 μg/ml at time intervals of 5 minutes between doses. The frequency and intensity of the uterine contractions were monitored via Powerlab software. Maximum contractions for both extracts were identified, recorded and the uterine strips samples at maximum contraction were selected and homogenized in order determine the role of prostaglandin F2α (PGF2α) in the mechanism of uterine contraction. Other than that, phosphorylated 42/44 (p42/44) of mitogen activated protein kinase (MAPK) expression was also detected via immunoblotting. The results showed that the maximum contraction induced by FDD was at the concentration of 320 μg/ml, whereas for FDA was at 960 μg/ml. Both FDD and FDA increased the intensity of uterine strips contractions and there were notable trend of increased PGF2α expression as well. Further analysis revealed that the uterine contractions involved the MAPK pathway through the phosphorylation of p42/44 protein. In conclusion, Ficus deltoidea of both variants have the ability to stimulate uterine contraction through the mechanism of MAPK pathway.
format	Article
author	Farah Wahida Ibrahim, Nor Idayu Derased, Umi Romaizatul Amiera Zainudin, Nor Fadilah Rajab,
spellingShingle	Farah Wahida Ibrahim, Nor Idayu Derased, Umi Romaizatul Amiera Zainudin, Nor Fadilah Rajab, Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
author_facet	Farah Wahida Ibrahim, Nor Idayu Derased, Umi Romaizatul Amiera Zainudin, Nor Fadilah Rajab,
author_sort	Farah Wahida Ibrahim,
title	Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
title_short	Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
title_full	Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
title_fullStr	Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
title_full_unstemmed	Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions
title_sort	mechanism identification of ficus deltoidea aqueous extract in rat uterine contractions
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12943/ http://journalarticle.ukm.my/12943/ http://journalarticle.ukm.my/12943/1/24032-76983-1-PB.pdf
first_indexed	2023-09-18T20:03:44Z
last_indexed	2023-09-18T20:03:44Z
_version_	1777407020059590656

Mechanism identification of Ficus Deltoidea aqueous extract in rat uterine contractions

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