Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana

Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which...

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Main Authors:	Lyena Watty Zuraine Ahmad, Zarina Zainuddin, Mohamad Osman, Azhar Mohamad, Norrizah Jaafar Sidik
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13080/ http://journalarticle.ukm.my/13080/ http://journalarticle.ukm.my/13080/1/05%20Lyena%20Watty%20Zuraine%20Ahmad.pdf

id	ukm-13080
recordtype	eprints
spelling	ukm-130802019-06-22T00:22:06Z http://journalarticle.ukm.my/13080/ Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana Lyena Watty Zuraine Ahmad, Zarina Zainuddin, Mohamad Osman, Azhar Mohamad, Norrizah Jaafar Sidik, Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. Penerbit Universiti Kebangsaan Malaysia 2019-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13080/1/05%20Lyena%20Watty%20Zuraine%20Ahmad.pdf Lyena Watty Zuraine Ahmad, and Zarina Zainuddin, and Mohamad Osman, and Azhar Mohamad, and Norrizah Jaafar Sidik, (2019) Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana. Sains Malaysiana, 48 (2). pp. 301-307. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU.
format	Article
author	Lyena Watty Zuraine Ahmad, Zarina Zainuddin, Mohamad Osman, Azhar Mohamad, Norrizah Jaafar Sidik,
spellingShingle	Lyena Watty Zuraine Ahmad, Zarina Zainuddin, Mohamad Osman, Azhar Mohamad, Norrizah Jaafar Sidik, Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
author_facet	Lyena Watty Zuraine Ahmad, Zarina Zainuddin, Mohamad Osman, Azhar Mohamad, Norrizah Jaafar Sidik,
author_sort	Lyena Watty Zuraine Ahmad,
title	Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
title_short	Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
title_full	Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
title_fullStr	Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
title_full_unstemmed	Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana
title_sort	effects of n-methyl-n-nitrosourea on seed germination of stevia rebaudiana
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13080/ http://journalarticle.ukm.my/13080/ http://journalarticle.ukm.my/13080/1/05%20Lyena%20Watty%20Zuraine%20Ahmad.pdf
first_indexed	2023-09-18T20:04:03Z
last_indexed	2023-09-18T20:04:03Z
_version_	1777407039471878144

Effects of N-Methyl-N-Nitrosourea on seed germination of Stevia rebaudiana

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